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Nerve Growth Factor and Neurotrophin-3 Differentially Regulate the Proliferation and Survival of Developing Rat Brain Oligodendrocytes
- Source :
- The Journal of Neuroscience. 16:6433-6442
- Publication Year :
- 1996
- Publisher :
- Society for Neuroscience, 1996.
-
Abstract
- There is increasing evidence that the neurotrophins, particularly nerve growth factor (NGF) and neurotrophin-3 (NT-3), play a role in the regulation of glial development in the CNS. Recent studies have shown that the proliferation of optic nerve-derived O2A progenitors (OLPs) is potentiated by NT-3 in combination with platelet-derived growth factor, whereas NT-3 alone supports the survival of their differentiated progeny (Barres et al., 1994). In this study, we have examined the expression of the high-affinity neurotrophin receptors (trks) and the low-affinity nerve growth factor receptor p75 in developing oligodendrocytes (OLs). In addition, we have examined the effects of NGF and NT-3 on proliferation and survival of OLPs and OLs, respectively. TrkC, the high-affinity NT-3 receptor, and trkA, the high-affinity NGF receptor, are both expressed from the early OLP through the mature OL stage. The truncated form of trkB, lacking the tyrosine kinase domain, and the low-affinity neurotrophin receptor p75 are expressed at low levels in OLPs and are upregulated in mature OLs. NGF and NT-3 both induced the phosphorylation of mitogen-activated protein kinase (MAPK) in OLPs and in OLs. In both OLPs and OLs, NT-3 sustained the activation of MAPK more than NGF. NT-3 enhanced the proliferation of OLPs and supported the survival of OLs. By contrast, unless coadministered with FGF-2, NGF did not exhibit mitogenic effects on OLPs but did enhance the survival of differentiated OLs. Our data demonstrate the presence of functional trkA and trkC in developing OLs and indicate that both NGF and NT-3 have a broad spectrum of developmental actions on cells of the OL lineage.
- Subjects :
- medicine.medical_specialty
Cell Survival
medicine.medical_treatment
Tropomyosin receptor kinase B
Neurotrophin-3
Tropomyosin receptor kinase A
Tropomyosin receptor kinase C
Internal medicine
Cell Adhesion
medicine
Animals
Nerve Growth Factors
Dose-Response Relationship, Drug
biology
General Neuroscience
Growth factor
Brain
Articles
Immunohistochemistry
Rats
Cell biology
Oligodendroglia
stomatognathic diseases
Nerve growth factor
Endocrinology
nervous system
biology.protein
Tyrosine kinase
Neurotrophin
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....b8e13c2966d9faffeeaf4655a896fc05