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Molecular characterization and function analysis of a nucleotide excision repair gene Rad23 from Litopenaeus vannamei after Vibrio alginolyticus challenge
- Source :
- Fish & Shellfish Immunology. 83:190-204
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Nucleotide excision repair (NER) removes many different types of DNA lesions, and NER related host factors are reported to aid recovery steps during viral integration. Here, we report the identification and characterization of a DNA repair gene Rad23 from Litopenaeus vannamei and explore its role in innate immunity of crustaceans. LvRad23 contains a1149 bp open reading frame (ORF) which encodes a 382 amino acids protein with predicted theoretical isoelectric point of 4.21. LvRad23 was ubiquitously expressed in the muscle, eyestalk, gill, stomach, heart, legs, intestine, and hepatopancreas in order from high to low and LvRad23 protein was showed to be located in the cytoplasm of Drosophila S2 cells. The homology analysis showed that it has a high sequence homology with Rad23 protein from Marsupenaeus japonicus. Vibrio alginolyticus challenge induced a remarkable up-regulation of LvRad23 mRNA in hepatopancreas. Knocking down LvRad23can interfere the NER pathway by down regulating the expression of replication protein A (RPA) and proliferating cell nuclear antigen (PCNA). However it didn't cause any significant difference on total hemocyte count (THC) between LvRad23-silenced and non-silenced group.LvRad23-silenced then challenge with V. alginolyticus inducing high level of reactive oxygen species (ROS) and DNA damage in hemolymph. As well as decreased THC, which seriously diminished the innate immune system of L. vannamei. Meanwhile, the NER pathway was reactived by enhancing the expression of LvRad23 and promoting the production of LvPCNA to resist apoptosis and maintain proliferation of hemolymph cells in the later stage. Our results suggest that LvRad23 plays a vital role in shrimp specific immune response to V. alginolytcus through its participation in NER pathway.
- Subjects :
- 0301 basic medicine
DNA, Complementary
DNA Repair
DNA damage
Aquatic Science
Arthropod Proteins
03 medical and health sciences
Penaeidae
Animals
Environmental Chemistry
Replication protein A
Gene
Vibrio alginolyticus
Innate immune system
030102 biochemistry & molecular biology
biology
General Medicine
biology.organism_classification
Cell biology
Proliferating cell nuclear antigen
DNA-Binding Proteins
Open reading frame
DNA Repair Enzymes
030104 developmental biology
biology.protein
Nucleotide excision repair
Subjects
Details
- ISSN :
- 10504648
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Fish & Shellfish Immunology
- Accession number :
- edsair.doi.dedup.....b8e53bb01a2fde0cff4a20bfbea34395