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Role of SOCS3 in enhanced acute-phase protein genes by neonatal macrophages in response to IL-6
- Source :
- Journal of Microbiology, Immunology and Infection, Vol 54, Iss 2, Pp 206-212 (2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Background Interleukin 6 (IL-6) induce the inflammatory response directly related with the morbidity and mortality of neonatal. Here we aimed to explore the mechanism of IL-6 in neonatal inflammatory response by studying the IL-6/STAT3 signaling pathway. Methods Cord blood samples from health term neonatal and peripheral venous blood from health volunteers were collected. The monocytes of adults and cord blood were isolated and induced into macrophages. Then the macrophages were pretreated with or without MG132 before IL-6 stimulation. Proteins were analyzed by Western blot, mRNA by real time PCR and membrane molecule by flow cytometry. Results The acute phase protein gene expression in neonatal macrophages after stimulated with IL-6 were higher than that in adult. Significantly enhanced phosphorylation of STAT3 was seen in neonatal macrophages. Both mRNA and protein expression of SOCS3 in neonatal macrophages were lower than that in adult. After pretreated with MG132, the expression of SOCS3 protein was increased which lead to attenuate the STAT3 phosphorylation and APP gene expression. Conclusion Neonatal exhibit an enhanced expression of downstream target genes and IL-6/STAT3 signal pathway which is related with the diminished SOCS3. This provides a new sight into inflammatory responses in neonatal.
- Subjects :
- Adult
STAT3 Transcription Factor
0301 basic medicine
Microbiology (medical)
030106 microbiology
Microbiology
Monocytes
STAT3
Andrology
Young Adult
03 medical and health sciences
0302 clinical medicine
Western blot
Gene expression
Humans
Immunology and Allergy
Medicine
SOCS3
RNA, Messenger
030212 general & internal medicine
Interleukin 6
IL-6
General Immunology and Microbiology
biology
Neonatal sepsis
medicine.diagnostic_test
Interleukin-6
business.industry
Macrophages
Acute-phase protein
General Medicine
medicine.disease
QR1-502
Infectious Diseases
Gene Expression Regulation
Suppressor of Cytokine Signaling 3 Protein
Cord blood
biology.protein
business
Acute-Phase Proteins
Signal Transduction
Subjects
Details
- ISSN :
- 16841182
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Journal of Microbiology, Immunology and Infection
- Accession number :
- edsair.doi.dedup.....b91b3c8ecf09040656535a898428290b