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Studies on the peparative capability of the horizontal flow-through coil planet centrifuge and high-performance liquid chromatography in the separation of polar compounds from Oxytropis ochrocephala Bunge
- Source :
- Journal of Chromatography A. 538:219-225
- Publication Year :
- 1991
- Publisher :
- Elsevier BV, 1991.
-
Abstract
- Horizontal flow-through coil planet centrifuge (CPC) and high-performance liquid chromatography (HPLC) techniques were used for separation of polar compounds from a crude ethanol extract of Oxytropis ochrocephala Bunge, a poisonous legume plant widely distributed in northwestern China. The performance of these two chromatographic methods was compared in terms of column efficiency, peak resolution, separation time, sample loading capacity, etc. The results indicated that two polar compounds in the crude extract were equally well separated by these two methods. HPLC gave comparable peak resolution in shorter separation time while its sample loading capacity was limited to the mg range. The CPC method required a long separation time, but yielded a higher purity of fractions with a much greater capacity.
- Subjects :
- Centrifuge
Plants, Medicinal
Chromatography
Resolution (mass spectrometry)
Chemistry
Chemical polarity
Organic Chemistry
Analytical chemistry
Centrifugation
Fabaceae
General Medicine
Oxytropis ochrocephala
Biochemistry
High-performance liquid chromatography
Analytical Chemistry
Plants, Toxic
Countercurrent chromatography
Electromagnetic coil
Glycosides
Horizontal flow
Chromatography, High Pressure Liquid
Chromatography, Liquid
Subjects
Details
- ISSN :
- 00219673
- Volume :
- 538
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography A
- Accession number :
- edsair.doi.dedup.....b93898ee412f871f1d953de6880680e7