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ECM-Body: A Cell-Free 3D Biomimetic Scaffold Derived from Intact Planarian Body
- Source :
- Zoological Science. 37:307
- Publication Year :
- 2020
- Publisher :
- Zoological Society of Japan, 2020.
-
Abstract
- Extracellular matrix (ECM) plays key roles in shaping fates of stem cells, not only by providing a suitable niche but also by mediating physical and biochemical cues. Despite intensive investigations on regeneration, the roles of ECM in fate determination of stem cells in animals with great regenerative potency, such as planarian, have remained unclear. Here, we developed a method for decellularizing and isolating extracellular matrix from planarians. Although the isolated scaffold appears translucent, it contains all the internal features resembling those of the structure of intact planarians, and we thus called it the "ECM-body". Nuclear staining demonstrated that the ECM-body contains very few or no remaining cells. Histological sections displayed well-preserved morphological integrity of the specimen. Scanning electron microscopy showed a porous surface on the ECM-body, potentially suitable for housing cells. Furthermore, our preliminary experiment suggested that ECM-body can be utilized as a biomimetic scaffold for cell culture as it may support survival of injected neoblasts.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Scaffold
Decellularization
Cell-Free System
Tissue Scaffolds
Regeneration (biology)
Planarians
Biology
biology.organism_classification
010603 evolutionary biology
01 natural sciences
Extracellular Matrix
Cell biology
Extracellular matrix
03 medical and health sciences
030104 developmental biology
Tissue engineering
Biomimetic Materials
Cell culture
Planarian
Animals
Animal Science and Zoology
Stem cell
Subjects
Details
- ISSN :
- 02890003
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Zoological Science
- Accession number :
- edsair.doi.dedup.....b939c69fd15d739c411d40616895dc58