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Proteomic analysis reveals the mechanism of different environmental stress-induced tolerance of Pseudomonas aeruginosa to monochloramine disinfection
- Source :
- Journal of Hazardous Materials. 417:126082
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Although drinking water disinfection proved to be an effective strategy to eliminate many pathogens, bacteria can still show disinfection tolerance in drinking water distribution systems. To date, the molecular mechanisms on how environmental stress affects the tolerance of Pseudomonas aeruginosa to monochloramine are not well understood. Here, we investigated how three stress conditions, namely starvation, low temperature, and starvation combined with low temperature, affected the monochloramine tolerance of Pseudomonas aeruginosa, an opportunistic pathogen commonly found in drinking water distribution systems. All stress conditions significantly promoted monochloramine tolerance, among which starvation had the most drastic effects. Proteomic analyses suggested that the three conditions not only triggered a positive antioxidant defense against oxidative damages but also prepared the bacteria to employ a passive defense mechanism against disinfectants via dormancy. Moreover, the expression of antioxidant enzymes reached the maximum under the starvation condition and further low temperature treatment had little effect on bacterial response to oxidative stress. Instead, we found further treatment of the starved cells with low temperature decreased the osmotic stress response and the stringent response, which generally play pivotal roles in disinfection tolerance. Taken together, these findings shed light on how abiotic factors influence the bacterial disinfection tolerance and will aid design of efficient strategies to eliminate Pseudomonas aeruginosa from drinking water.
- Subjects :
- Proteomics
Environmental Engineering
Antioxidant
Stringent response
Health, Toxicology and Mutagenesis
medicine.medical_treatment
0211 other engineering and technologies
02 engineering and technology
Oxidative phosphorylation
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Microbiology
medicine
Environmental Chemistry
Waste Management and Disposal
0105 earth and related environmental sciences
Abiotic component
021110 strategic, defence & security studies
biology
Chemistry
Pseudomonas aeruginosa
Drinking Water
Chloramines
biology.organism_classification
Pollution
Disinfection
Dormancy
Bacteria
Oxidative stress
Disinfectants
Subjects
Details
- ISSN :
- 03043894
- Volume :
- 417
- Database :
- OpenAIRE
- Journal :
- Journal of Hazardous Materials
- Accession number :
- edsair.doi.dedup.....b9427e64887c1ade6bedd47c1621d3ca