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Adhesion to the host cell surface is sufficient to mediate Listeria monocytogenes entry into epithelial cells
- Source :
- Molecular Biology of the Cell
- Publication Year :
- 2017
- Publisher :
- The American Society for Cell Biology, 2017.
-
Abstract
- Listeria monocytogenes invades epithelial cells by binding to the host cell receptor E-cadherin, a component of the adherens junction. E-cadherin serves primarily as an adhesive to mediate bacterial invasion; the canonical E-cadherin/catenin/F-actin complex is not required for this process.<br />The intestinal epithelium is the first physiological barrier breached by the Gram-positive facultative pathogen Listeria monocytogenes during an in vivo infection. Listeria monocytogenes binds to the epithelial host cell receptor E-cadherin, which mediates a physical link between the bacterium and filamentous actin (F-actin). However, the importance of anchoring the bacterium to F-actin through E-cadherin for bacterial invasion has not been tested directly in epithelial cells. Here we demonstrate that depleting αE-catenin, which indirectly links E-cadherin to F-actin, did not decrease L. monocytogenes invasion of epithelial cells in tissue culture. Instead, invasion increased due to increased bacterial adhesion to epithelial monolayers with compromised cell–cell junctions. Furthermore, expression of a mutant E-cadherin lacking the intracellular domain was sufficient for efficient L. monocytogenes invasion of epithelial cells. Importantly, direct biotin-mediated binding of bacteria to surface lipids in the plasma membrane of host epithelial cells was sufficient for uptake. Our results indicate that the only requirement for L. monocytogenes invasion of epithelial cells is adhesion to the host cell surface, and that E-cadherin–mediated coupling of the bacterium to F-actin is not required.
- Subjects :
- 0301 basic medicine
Cell Culture Techniques
Biology
medicine.disease_cause
Filamentous actin
Madin Darby Canine Kidney Cells
03 medical and health sciences
Tissue culture
0302 clinical medicine
Dogs
Listeria monocytogenes
Bacterial Proteins
Cell Line, Tumor
medicine
Cell Adhesion
Animals
Humans
Receptor
Molecular Biology
Pathogen
Host cell surface
Cell Membrane
Epithelial Cells
Cell Biology
Adhesion
Articles
Cadherins
Intestinal epithelium
Actins
Cell biology
030104 developmental biology
Intercellular Junctions
Cell Biology of Disease
Antigens, Surface
030217 neurology & neurosurgery
alpha Catenin
Subjects
Details
- Language :
- English
- ISSN :
- 19394586 and 10591524
- Volume :
- 28
- Issue :
- 22
- Database :
- OpenAIRE
- Journal :
- Molecular Biology of the Cell
- Accession number :
- edsair.doi.dedup.....b9640b11b9f5f4edbce811dc53cf4d11