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Ginseng-Sanqi-Chuanxiong (GSC) Extracts Ameliorate Diabetes-Induced Endothelial Cell Senescence through Regulating Mitophagy via the AMPK Pathway
- Source :
- Oxidative Medicine and Cellular Longevity, Vol 2020 (2020), Oxidative Medicine and Cellular Longevity
- Publication Year :
- 2020
- Publisher :
- Hindawi Limited, 2020.
-
Abstract
- Vascular endothelial senescence induced by high glucose and palmitate (HG/PA) contributes to endothelial dysfunction, which leads to diabetic cardiovascular complications. Reduction of endothelial senescence may attenuate these pathogenic processes. This study is aimed at determining whether Ginseng-Sanqi-Chuanxiong (GSC) extracts, traditional Chinese medicine, can ameliorate human aortic endothelial cell (HAEC) senescence under HG/PA-stressed conditions and further explore the underlying mechanism. We found that GSC extracts significantly increased antisenescent activity by reducing the HG/PA-induced mitochondrial ROS (mtROS) levels in senescent HAECs. GSC extracts also induced cellular mitophagy formation, which mediated the effect of GSC extracts on mtROS reduction. Apart from this, the data showed that GSC extracts stimulated mitophagy via the AMPK pathway, and upon inhibition of AMPK by pharmacological and genetic inhibitors, GSC extract-mediated mitophagy was abolished which further led to reverse the antisenescence effect. Taken together, these data suggest that GSC extracts prevent HG/PA-induced endothelial senescence and mtROS production by mitophagy regulation via the AMPK pathway. Thus, the induction of mitophagy by GSC extracts may provide a novel therapeutic candidate for cardiovascular protection in metabolic syndrome.
- Subjects :
- Senescence
Mitochondrial ROS
endocrine system
Aging
animal structures
Article Subject
Cell Survival
Palmitic Acid
AMP-Activated Protein Kinases
Models, Biological
Biochemistry
Ginseng
Diabetes mellitus
Mitophagy
Autophagy
Diabetes Mellitus
medicine
Humans
RNA, Small Interfering
Endothelial dysfunction
Cellular Senescence
Quinazolinones
QH573-671
Plant Extracts
Chemistry
fungi
Endothelial Cells
AMPK
Cell Biology
General Medicine
medicine.disease
Mitochondria
Cell biology
Endothelial stem cell
Glucose
embryonic structures
Cytology
Research Article
Drugs, Chinese Herbal
Signal Transduction
Subjects
Details
- ISSN :
- 19420994 and 19420900
- Volume :
- 2020
- Database :
- OpenAIRE
- Journal :
- Oxidative Medicine and Cellular Longevity
- Accession number :
- edsair.doi.dedup.....b9a49bee2350af4e181b598c463f79d0