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Hydrolysis of 2′,3′-cyclic adenosine monophosphate and 3′,5′-cyclic adenosine monophosphate in subcellular fractions of normal and neoplastic mouse spleen
- Source :
- Life Sciences, 15(3), 491-499
- Publication Year :
- 1974
- Publisher :
- Elsevier BV, 1974.
-
Abstract
- A comparison has been made between the capacity to hydrolyse 2′,3′-cyclic adenosine monophosphate and 3′,5′-cyclic adenosine monophosphate in subcellular fractions of normal and neoplastic (lymphosarcoma) spleen of C 57 BL mice. The effect of X-irradiation on these activities was tested. Subcellular fractionation of normal and lymphosarcoma spleen points to a different overall localization of the enzymes. The 2′,3′-cyclic nucleotide phosphodiesterase (2′,3′-cAMPase) has its highest specific activity in the particulate fractions of the cell, while the data on 3′,5′-cyclic nucleotide phosphodiesterase (3′,5′-cAMPase) show the highest activity in the soluble fraction. The 2′,3′-cAMPase activity is higher in the tumor as compared to the normal tissue, while the opposite holds for 3′,5′-cAMPase. Total body irradiation of normal mice with a dose of 600 rads of X-rays, results in a clear drop in 2′,3′-cAMPase 48 hours after the exposure. The 3′,5′-cAMPase is hardly affected at this time. Neither imidazol nor Mg ++ has any influence on the 2′,3′-cAMPase. The pH optimum for 3′,5′-cAMPase and 2′,3′-cAMPase appears to be 7.7 and 6.2 respectively. This report suggests a no-identity of the two enzymes in mouse spleen, a situation different from that found in certain plants.
- Subjects :
- Adenosine monophosphate
Spleen
General Biochemistry, Genetics and Molecular Biology
Mice
chemistry.chemical_compound
Cyclic AMP
medicine
Animals
Cyclic adenosine monophosphate
General Pharmacology, Toxicology and Pharmaceutics
chemistry.chemical_classification
Hydrolysis
Splenic Neoplasms
Phosphodiesterase
General Medicine
Metabolism
Mice, Inbred C57BL
medicine.anatomical_structure
Enzyme
chemistry
Biochemistry
3',5'-Cyclic-AMP Phosphodiesterases
Specific activity
Cell fractionation
2',3'-Cyclic-Nucleotide Phosphodiesterases
Subcellular Fractions
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Life Sciences
- Accession number :
- edsair.doi.dedup.....b9c61a2d1fc86f1e14474ae77aa6ca7d