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Eryngium Billardieri Induces Apoptosis via Bax Gene Expression in Pancreatic Cancer Cells
- Source :
- Advanced Pharmaceutical Bulletin, Advanced Pharmaceutical Bulletin, Vol 8, Iss 4, Pp 667-674 (2018)
- Publication Year :
- 2018
- Publisher :
- Maad Rayan Publishing Company, 2018.
-
Abstract
- Purpose: Pancreatic adenocarcinoma has a high prevalence all over the world. Most of the therapeutic approaches failed as a result of tumor invasion and rapid metastasis. Several natural plants have been shown to have promising therapeutic effects. Thus, the aim of this study was to investigate the cytotoxic activity of Eryngium billardieri against PANC-1 cancer cell lines. Methods: Dimethylthiazole diphenyltetrazolium bromide assay (MTT assay) and flow cytometry were used to assess the cytotoxicity of E. billardieri extracts against PANC-1 cancer cell lines. Quantitative Polymerase Chain Reaction (qPCR) was conducted to investigate the expression levels of Bcl2- associated X protein (BAX) and cyclin D1. Results: The results of the MTT assay showed that E. billardieri extracts had cytotoxic effects on PANC- 1 cancer cell lines. Moreover, the findings from the gene expression confirmed the over expression of Bax, and under expression of cyclin D1 following treatment with dichloromethane (DCM) and n-hexane (n- hex) extracts in cancer cells (P < 0.05). Interestingly, the flow cytometry results showed that DCM and n- hex extracts of E. billardieri induced apoptosis in PANC- 1 cancer cell lines. Conclusion: The results of this study demonstrated that DCM and n- hex extracts of E. billardieri significantly induce apoptosis by increasing Bax and decreasing cyclin D1 mRNA expression. Therefore, E. billardieri may be regarded as a novel approach for treatment of pancreatic cancer as a result of its promising apoptotic and cytotoxic properties.
- Subjects :
- 0301 basic medicine
Eryngium
Pharmaceutical Science
Adenocarcinoma
03 medical and health sciences
0302 clinical medicine
Cyclin D1
Pancreatic cancer
medicine
Cytotoxic T cell
MTT assay
General Pharmacology, Toxicology and Pharmaceutics
Cytotoxicity
Pancreas
Chemistry
lcsh:RM1-950
medicine.disease
Molecular biology
lcsh:Therapeutics. Pharmacology
030104 developmental biology
Real-time polymerase chain reaction
Bax
Apoptosis
030220 oncology & carcinogenesis
Cancer cell
Research Article
Subjects
Details
- ISSN :
- 22517308 and 22285881
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Advanced Pharmaceutical Bulletin
- Accession number :
- edsair.doi.dedup.....b9c99bb29b6e006b04340d61e37ae630