Back to Search
Start Over
Effect of chronic intermittent hypoxia on leptin and leptin receptor protein expression in the carotid body
- Source :
- Brain Research. 1513:51-60
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- This study was done to investigate whether chronic intermittent hypoxia (CIH) induced changes in leptin and leptin receptor protein levels, and known downstream mediators of leptin receptor signaling in the carotid body. Rats were subjected to CIH (120s normoxia, 80s hypoxia) or normoxia for 8h/day to either short term (7 days) or long term CIH exposure (95 days). After both 7 and 95 days of CIH, carotid body leptin protein expression was decreased, while protein levels of the long form leptin receptor (OB-Rb) were elevated. On the other hand, protein expression levels of the short form leptin receptor (OB-R100) were unchanged. Furthermore, phosphorylated signal transducer and activator of transcription 3 (pSTAT3) protein levels were found to be significantly decreased at only the 7 day period. On the other hand, suppressor of cytokine signaling 3 (SOCS3) protein levels were elevated at only the 7 day period, while phosphorylated extracellular-signal-regulated kinase 1/2 (pERK1/2) was elevated only at the 95 day period. In both the normoxia and the CIH groups, carotid body leptin was decreased at the 95 day period compared to 7 days. However, OB-Rb or Ob-R100 protein levels were not changed in the normoxic or CIH group at either time point. Furthermore, pSTAT3 protein levels were found to be significantly higher, while SOCS3 levels were significantly lower in the 95 day CIH group compared to the 7 day CIH group. Taken together, these data indicate that CIH induces changes in leptin and leptin downstream signaling proteins within the carotid bodies which may contribute to alterations in carotid chemoreceptor sensitivity.
- Subjects :
- Leptin
Male
STAT3 Transcription Factor
medicine.medical_specialty
Time Factors
MAP Kinase Signaling System
Suppressor of Cytokine Signaling Proteins
Biology
Rats, Sprague-Dawley
Internal medicine
medicine
Animals
SOCS3
Hypoxia
Receptor
STAT3
Molecular Biology
Carotid Body
Leptin receptor
Kinase
General Neuroscience
digestive, oral, and skin physiology
Hypoxia (medical)
Rats
Disease Models, Animal
medicine.anatomical_structure
Endocrinology
Gene Expression Regulation
Suppressor of Cytokine Signaling 3 Protein
biology.protein
Receptors, Leptin
Carotid body
Neurology (clinical)
medicine.symptom
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 1513
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....ba40ba93b091262d82b94ac892c5a5fd