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16S rRNA of Mucosal Colon Microbiome and CCL2 Circulating Levels Are Potential Biomarkers in Colorectal Cancer
- Source :
- International Journal of Molecular Sciences, Volume 22, Issue 19, International journal of molecular sciences, 22 (2021). doi:10.3390/ijms221910747, info:cnr-pdr/source/autori:Nardelli C.; Granata I.; Nunziato M.; Setaro M.; Carbone F.; Zulli C.; Pilone V.; Capoluongo E.D.; De Palma G.D.; Corcione F.; Matarese G.; Salvatore F.; Sacchetti L./titolo:16s rRNA of mucosal colon microbiome and CCL2 circulating levels are potential biomarkers in colorectal cancer/doi:10.3390%2Fijms221910747/rivista:International journal of molecular sciences (Print)/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume:22, International Journal of Molecular Sciences, Vol 22, Iss 10747, p 10747 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Colorectal cancer (CRC) is one of the most common malignancies in the Western world and intestinal dysbiosis might contribute to its pathogenesis. The mucosal colon microbiome and C-C motif chemokine 2 (CCL2) were investigated in 20 healthy controls (HC) and 20 CRC patients using 16S rRNA sequencing and immunoluminescent assay, respectively. A total of 10 HC subjects were classified as overweight/obese (OW/OB_HC) and 10 subjects were normal weight (NW_HC)<br />15 CRC patients were classified as OW/OB_CRC and 5 patients were NW_CRC. Results: Fusobacterium nucleatum and Escherichia coli were more abundant in OW/OB_HC than in NW_HC microbiomes. Globally, Streptococcus intermedius, Gemella haemolysans, Fusobacterium nucleatum, Bacteroides fragilis and Escherichia coli were significantly increased in CRC patient tumor/lesioned tissue (CRC_LT) and CRC patient unlesioned tissue (CRC_ULT) microbiomes compared to HC microbiomes. CCL2 circulating levels were associated with tumor presence and with the abundance of Fusobacterium nucleatum, Bacteroides fragilis and Gemella haemolysans. Our data suggest that mucosal colon dysbiosis might contribute to CRC pathogenesis by inducing inflammation. Notably, Fusobacterium nucleatum, which was more abundant in the OW/OB_HC than in the NW_HC microbiomes, might represent a putative link between obesity and increased CRC risk.
- Subjects :
- Male
Chemokine
obesity
Colorectal cancer
microbiome
Colorectal Neoplasm
CCL2 [chemokine (C‐C motif) ligand 2]
Pathogenesis
RNA, Ribosomal, 16S
Biology (General)
Intestinal Mucosa
Chemokine CCL2
Spectroscopy
biology
CCL2 [chemokine (C-C motif) ligand 2]
General Medicine
Middle Aged
Computer Science Applications
Chemistry
Female
Bacteroides fragilis
Colorectal Neoplasms
Case-Control Studie
CCL2
Human
QH301-705.5
16s sequencing
colorectal cancer
Streptococcus intermedius
Article
Catalysis
Microbiology
Inorganic Chemistry
stomatognathic system
medicine
Humans
Microbiome
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
Aged
Bacteria
business.industry
Organic Chemistry
Biomarker
mucosal colon microbiome
biology.organism_classification
medicine.disease
digestive system diseases
Gastrointestinal Microbiome
stomatognathic diseases
Case-Control Studies
biology.protein
16S rRNA gene
Fusobacterium nucleatum
business
Dysbiosis
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....ba4716ba4bf5ad6fa3e0825ea06c3c79
- Full Text :
- https://doi.org/10.3390/ijms221910747