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Enhancement of gallic acid-induced human pulmonary fibroblast cell death by N-acetyl cysteine and L-buthionine sulfoximine
- Source :
- Human & Experimental Toxicology. 30:992-999
- Publication Year :
- 2010
- Publisher :
- SAGE Publications, 2010.
-
Abstract
- Gallic acid (GA) has various biological properties including anti-cancer effect. However, little is known about the toxicological effect of GA in primary normal cells. Here, we evaluated the effects of GA on human pulmonary fibroblast (HPF) cells in relation to reactive oxygen species (ROS) and glutathione (GSH). GA inhibited the growth of HPF cells at 24 hours in a dose-dependent manner. GA also induced HPF cell death, which was accompanied by the loss of mitochondrial membrane potential (MMP; ΔΨm). GA increased ROS levels including O2•- and GSH-depleted cell numbers in HPF cells at 24 hours. Treatment with 2 mM N-acetyl-cysteine (NAC) intensified growth inhibition and death in GA-treated HPF cells. NAC decreased ROS levels and increased GSH depletion in these cells. Treatment with 10 μM L-buthionine sulfoximine (BSO) also enhanced growth inhibition and death in GA-treated HPF cells. BSO increased ROS levels and GSH depletion in these cells. In conclusion, GA-induced HPF cell death was accompanied by ROS increase and GSH depletion. The changes of ROS and GSH levels by NAC and BSO appeared to affect cell growth and death in GA-treated HPF cells.
- Subjects :
- Programmed cell death
animal structures
Health, Toxicology and Mutagenesis
Cell
Cell Culture Techniques
Biology
Toxicology
Cell Line
chemistry.chemical_compound
Methionine
Gallic Acid
medicine
Anticarcinogenic Agents
Humans
Gallic acid
Fibroblast
Lung
Cell Proliferation
Membrane Potential, Mitochondrial
chemistry.chemical_classification
Reactive oxygen species
Cell Death
Dose-Response Relationship, Drug
Cell growth
Drug Synergism
General Medicine
Glutathione
Fibroblasts
Molecular biology
Acetylcysteine
Cell biology
medicine.anatomical_structure
chemistry
Growth inhibition
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 14770903 and 09603271
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Human & Experimental Toxicology
- Accession number :
- edsair.doi.dedup.....ba62010ddb8ee5f43afd8f2dbf781d85