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Binge Alcohol Exposure Causes Neurobehavioral Deficits and GSK3β Activation in the Hippocampus of Adolescent Rats
- Source :
- Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
- Publication Year :
- 2017
-
Abstract
- Heavy alcohol exposure causes profound damage to the adolescent brain, particularly the hippocampus, which underlie some behavioral deficits. However, the underlying molecular mechanisms remain inconclusive. The current study sought to determine whether binge alcohol exposure affects the hippocampus-related behaviors and key signaling proteins that may mediate alcohol neurotoxicity in adolescent rats. Alcohol exposure reduced the number of both NeuN-positive and doublecortin-positive cells in the hippocampus. Alcohol also induced neurodegeneration which was confirmed by ultrastructural analysis by electronic microscopy and was accompanied with the activation of microglia. Binge alcohol exposure impaired spatial learning and memory which was evaluated by the Morris water maze. However, alcohol did not alter the spontaneous locomotor activity which was determined by the open field test. GSK3β is a multi-function serine/threonine protein kinase regulating both neuronal survival and neurogenesis and plays an important role in various neurodegenerative disorders. We have previously shown that GSK3β is a key mediator of alcohol-induced neuron apoptosis in the developing brain. We showed here binge alcohol exposure caused GSK3β activation by inducing dephosphorylation at Ser9 without affecting the phosphorylation of Tyr216 in the hippocampus. Thus, GSK3β may be involved in binge alcohol exposure-induced neuronal damage to the adolescent hippocampus.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Neurotoxicity Syndrome
Doublecortin Protein
Cell Survival
Neurogenesis
Spatial Learning
Morris water navigation task
Hippocampus
lcsh:Medicine
Apoptosis
Open field
Article
Binge Drinking
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Memory
Internal medicine
medicine
Animals
lcsh:Science
Neurons
Multidisciplinary
Glycogen Synthase Kinase 3 beta
Microglia
business.industry
Neurodegeneration
lcsh:R
Neurotoxicity
Brain
Neurodegenerative Diseases
medicine.disease
Rats
030104 developmental biology
Endocrinology
medicine.anatomical_structure
nervous system
lcsh:Q
Female
Neurotoxicity Syndromes
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....ba73973135a55f2faf5f037fc00c3e54