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Kzp Controls Canonical wnt8 Signaling to Modulate Dorsoventral Patterning during Zebrafish Gastrulation
- Source :
- Journal of Biological Chemistry. 285:42086-42096
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- During vertebrate embryonic development, the body axis formation requires the action of Wnt signals and their antagonists. Zygotic canonical wnt8 expression appears exclusively at the ventrolateral margin and mediates Wnt/β-catenin activities to promote posterior and ventral cell fate. However, the mechanisms involved in the initiation of zygotic wnt8 signals are poorly understood. Here, we identify a novel, maternally derived transcription factor, Kzp (Kaiso zinc finger-containing protein), as an important determinant for the initiation of zygotic Wnt signals in zebrafish. Kzp is a DNA-binding transcription factor that recognizes specific consensus DNA sequences, 5′-(t/a/g)t(a/t/g)nctgcca-3′, through zinc fingers and controls the initiation of zygotic wnt8 expression by directly binding to the wnt8 promoter during zebrafish embryonic development. Depletion of Kzp strongly dorsalized embryos, which was characterized by the expansion of dorsal gene expression. Overexpression of Kzp caused posteriorization. These phenotypes were highly similar to ones induced by wnt8 depletion or overexpression and were rescued by alteration of wnt8 activity. Thus, our results provide the first insight into the mechanism involved in the initiation of zygotic canonical Wnt signals by a maternally derived transcription factor.
- Subjects :
- Transcription, Genetic
Cellular differentiation
Biology
Cell fate determination
Biochemistry
Cell Movement
Animals
Humans
Molecular Biology
Transcription factor
Zebrafish
Body Patterning
DNA Primers
Genetics
Zinc finger
Wnt signaling pathway
Gene Expression Regulation, Developmental
Zinc Fingers
DNA
Gastrula
Cell Biology
Zebrafish Proteins
biology.organism_classification
Cell biology
Wnt Proteins
Gastrulation
Cytoskeletal Proteins
RNA
Maternal to zygotic transition
Additions and Corrections
Transcription Factors
Developmental Biology
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 285
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....ba77da163a6a2c787e58ef8e19f5aaa2