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Purification and Characterization of Recombinant Lipid Storage Protein-2 from Drosophila melanogaster
- Source :
- Protein & Peptide Letters. 15:1027-1032
- Publication Year :
- 2008
- Publisher :
- Bentham Science Publishers Ltd., 2008.
-
Abstract
- Lipid storage protein 2 (Lsd 2) is a conserved insect protein that belongs to the small PAT family of proteins. PAT proteins are found associated to the lipid droplets of adipocytes and play significant roles in the regulation of triacylglycerides metabolism. Here we describe the expression and purification of Lsd2, its reconstitution in lipoprotein particles, the location of putative lipid binding sites and its secondary structure. This study provides the starting point for future studies on the mechanism of function of Lsd2. The similarities and differences between Lsd1 and Lsd2, the only PAT proteins found in insects, are discussed.
- Subjects :
- animal structures
Lipolysis
Blotting, Western
Fat Body
Molecular Sequence Data
Biology
Peptide Mapping
Biochemistry
Article
Chromatography, Affinity
Protein Structure, Secondary
Structure-Activity Relationship
Structural Biology
Lipid droplet
Adipocytes
Animals
Drosophila Proteins
Amino Acid Sequence
Cloning, Molecular
Binding site
Peptide sequence
Protein secondary structure
Binding Sites
Circular Dichroism
General Medicine
biology.organism_classification
Recombinant Proteins
Drosophila melanogaster
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Electrophoresis, Polyacrylamide Gel
Function (biology)
Drosophila Protein
Lipoprotein
Subjects
Details
- ISSN :
- 09298665
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Protein & Peptide Letters
- Accession number :
- edsair.doi.dedup.....ba981c415b547259205f1649fb69c540
- Full Text :
- https://doi.org/10.2174/092986608785849191