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In vitro and in vivo toxicity evaluation of plant virus nanocarriers
- Source :
- Colloids and surfaces. B, Biointerfaces. 129
- Publication Year :
- 2014
-
Abstract
- The use of biological self-assembling materials, plant virus nanoparticles in particular, appears very intriguing as it allows a great choice of symmetries and dimensions, easy chemical and biological engineering of both surface and/or internal cavity as well as safe and rapid production in plants. In this perspective, we present an initial evaluation of the safety profile of two structurally different plant viruses produced in Nicotiana benthamiana L. plants: the filamentous Potato virus X and the icosahedral Tomato bushy stunt virus. In vitro haemolysis assay was used to test the cytotoxic effects, which could arise by pVNPs interaction with cellular membranes, while early embryo assay was used to evaluate toxicity and teratogenicity in vivo. Data indicates that these structurally robust particles, still able to infect plants after incubation in serum up to 24. h, have neither toxic nor teratogenic effects in vitro and in vivo. This work represents the first safety-focused characterization of pVNPs in view of their possible use as drug delivery carriers. © 2015 Elsevier B.V.
- Subjects :
- Nicotiana benthamiana
Plant virus nanoparticle
Chick Embryo
Plasmid
Tomato bushy stunt viru
Tombusvirus
Mice
Nanoparticle
Teratogenicity
Colloid and Surface Chemistry
Tomato bushy stunt virus
Nanotechnology
Drug Carrier
Drug Carriers
biology
Plant virus nanoparticles
food and beverages
Surfaces and Interfaces
General Medicine
Potato virus X
Haemolysis
Chicken
Biochemistry
Genetic Vector
Genetic Engineering
Biotechnology
Plasmids
Hemolysi
Potexviru
Genetic Vectors
In Vitro Techniques
Hemolysis
In vivo
Plant virus
Tobacco
Animals
Physical and Theoretical Chemistry
Toxicity
Animal
In Vitro Technique
fungi
biology.organism_classification
Virology
In vitro
Nanoparticles
Mice, Inbred C57BL
Potexvirus
Teratogenesis
Tombusviru
Nanocarriers
Chickens
Subjects
Details
- ISSN :
- 18734367
- Volume :
- 129
- Database :
- OpenAIRE
- Journal :
- Colloids and surfaces. B, Biointerfaces
- Accession number :
- edsair.doi.dedup.....baa4a7bb5573e64439355aa2318f1cc9