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A Method for Studying Calcium Dynamics in Transgenic Mice
- Source :
- PLoS biology, vol 2, iss 6, PLoS Biology, Vol 2, Iss 6, p e163 (2004), PLoS Biology
- Publication Year :
- 2004
- Publisher :
- eScholarship, University of California, 2004.
-
Abstract
- Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity-dependent molecular and cellular processes. Great hopes—for the measurement of population activity, in particular—have therefore been placed on calcium indicators derived from the green fluorescent protein and their expression in (selected) neuronal populations. Calcium transients can rise within milliseconds, making them suitable as reporters of fast neuronal activity. We here report the production of stable transgenic mouse lines with two different functional calcium indicators, inverse pericam and camgaroo-2, under the control of the tetracycline-inducible promoter. Using a variety of in vitro and in vivo assays, we find that stimuli known to increase intracellular calcium concentration (somatically triggered action potentials (APs) and synaptic and sensory stimulation) can cause substantial and rapid changes in FCIP fluorescence of inverse pericam and camgaroo-2.<br />Winfred Denk and colleagues succeed in generating transgenic mice that express one of two calcium indicators in their cells, creating a valuable tool to study neuronal activity
- Subjects :
- Gene Expression
Medical and Health Sciences
Calcium in biology
Transgenic
Green fluorescent protein
Mice
0302 clinical medicine
Biology (General)
0303 health sciences
education.field_of_study
Microscopy
Brain Mapping
Voltage-dependent calcium channel
General Neuroscience
Brain
Mus (Mouse)
Biological Sciences
Olfactory Bulb
3. Good health
Cell biology
Doxycycline
Neurological
General Agricultural and Biological Sciences
Research Article
Fluorescence Recovery After Photobleaching
Signal Transduction
QH301-705.5
Transgene
1.1 Normal biological development and functioning
Population
Biophysics
chemistry.chemical_element
Mice, Transgenic
Biology
Calcium
Genetics/Genomics/Gene Therapy
Transfection
General Biochemistry, Genetics and Molecular Biology
Retina
Fluorescence
03 medical and health sciences
Underpinning research
Animals
Humans
education
030304 developmental biology
Calcium metabolism
General Immunology and Microbiology
Agricultural and Veterinary Sciences
Neurosciences
Fluorescence recovery after photobleaching
Luminescent Proteins
Microscopy, Fluorescence
chemistry
Hela Cells
Calcium Channels
Generic health relevance
030217 neurology & neurosurgery
Photic Stimulation
Neuroscience
HeLa Cells
Developmental Biology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- PLoS biology, vol 2, iss 6, PLoS Biology, Vol 2, Iss 6, p e163 (2004), PLoS Biology
- Accession number :
- edsair.doi.dedup.....baa9b85c33f20a316218a93f667ba7d7