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Genomic analysis of Elizabethkingia species from aquatic environments: Evidence for potential clinical transmission
- Source :
- Current Research in Microbial Sciences, Vol 3, Iss, Pp 100083-(2022)
- Publication Year :
- 2022
- Publisher :
- Elsevier, 2022.
-
Abstract
- Refereed/Peer-reviewed Elizabethkingia species are ubiquitous in aquatic environments, colonize water systems in healthcare settings and are emerging opportunistic pathogens with reports surfacing in 25 countries across six continents. Elizabethkingia infections are challenging to treat, and case fatality rates are high. Chromosomal blaB, blaGOB and blaCME genes encoding carbapenemases and cephalosporinases are unique to Elizabethkingia spp. and reports of concomitant resistance to aminoglycosides, fluoroquinolones and sulfamethoxazole-trimethoprim are known. Here, we characterized whole-genome sequences of 94 Elizabethkingia isolates carrying multiple wide-spectrum metallo-β-lactamase (blaB and blaGOB) and extended-spectrum serine‑β-lactamase (blaCME) genes from Australian aquatic environments and performed comparative phylogenomic analyses against national clinical and international strains. qPCR was performed to quantify the levels of Elizabethkingia species in the source environments. Antibiotic MIC testing revealed significant resistance to carbapenems and cephalosporins but susceptibility to fluoroquinolones, tetracyclines and trimethoprim-sulfamethoxazole. Phylogenetics show that three environmental E. anophelis isolates are closely related to E. anophelis from Australian clinical isolates (∼36 SNPs), and a new species, E. umeracha sp. novel, was discovered. Genomic signatures provide insight into potentially shared origins and a capacity to transfer mobile genetic elements with both national and international isolates.
- Subjects :
- Microbiology (medical)
food.ingredient
medicine.drug_class
Elizabethkingia
Cephalosporin
Antibiotics
Biology
QH426-470
Microbiology
food
Immunology and Microbiology (miscellaneous)
Phylogenetics
medicine
Genetics
bacteria
Gene
Transmission (medicine)
transmission
pathogens
QR1-502
Infectious Diseases
isolates
Healthcare settings
Mobile genetic elements
aquatic environment
Subjects
Details
- Language :
- English
- ISSN :
- 26665174
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Current Research in Microbial Sciences
- Accession number :
- edsair.doi.dedup.....baaf1e5f05efd646d053bb95e40db4e3