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Cytoplasmic transfer in the mouse in conjunction with intracytoplasmic sperm injection
- Source :
- Reproductive biomedicine online. 8(1)
- Publication Year :
- 2004
-
Abstract
- Cytoplasmic transfer (CT) from a donor to a recipient oocyte has been used clinically to facilitate human pregnancies. Data reported here describe the first characterization of CT coincident with intracytoplasmic sperm injection in the mouse system. Sibling oocytes were used to transfer 2, 4, or 6 pl of ooplasm to a recipient egg along with a sperm head using piezo-actuated injection. Survival and fertilization after CT were comparable to controls at 2 pl and 4 pl, but survival was significantly reduced with 6 pl volumes. Development to the blastocyst stage was also inversely related to CT volume, with some decline beginning with the 4 pl CT group. However, some blastocysts did develop in all of the groups. The results are in contrast with human eggs, which tolerate larger CT volumes. Results indicate that the mouse system can be used to characterize the transfer of exogenous materials concomitant with sperm injection, provided that the CT volume is not excessive.
- Subjects :
- Male
Cytoplasm
Sperm Head
Cell Survival
medicine.medical_treatment
Biology
Intracytoplasmic sperm injection
Sperm injection
Andrology
Embryonic and Fetal Development
Mice
Human fertilization
medicine
Animals
Blastocyst
Sperm Injections, Intracytoplasmic
Cell survival
Cytoplasmic transfer
Obstetrics and Gynecology
Oocyte
Mice, Inbred C57BL
medicine.anatomical_structure
Reproductive Medicine
Mice, Inbred DBA
Oocytes
Female
Developmental Biology
Subjects
Details
- ISSN :
- 14726483
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Reproductive biomedicine online
- Accession number :
- edsair.doi.dedup.....bab16301fbe0624c1a8e5f5910e94ebd