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Biofunctionalized Calcium Phosphate Cement to Enhance the Attachment and Osteodifferentiation of Stem Cells Released from Fast-Degradable Alginate-Fibrin Microbeads
- Source :
- Tissue Engineering Part A. 18:1583-1595
- Publication Year :
- 2012
- Publisher :
- Mary Ann Liebert Inc, 2012.
-
Abstract
- Stem cell-encapsulating microbeads could be mixed into a paste such as calcium phosphate cement (CPC), where the microbeads could protect the cells from the mixing and injection forces. After being placed, the microbeads could quickly degrade to release the cells throughout the scaffold, while creating macropores. The objectives of this study were to (1) construct alginate-fibrin microbeads encapsulating human umbilical cord mesenchymal stem cells (hUCMSCs) embedded in the surface of novel biofunctionalized CPC and (2) investigate microbead degradation, cell release, and osteodifferentiation on CPC. Hydrogel microbeads were fabricated that encapsulated hUCMSCs at 1×10(6) cells/mL. CPC was biofunctionalized with fibronectin (Fn) and Arg-Gly-Asp (RGD). Four scaffolds were tested: CPC control, CPC mixed with Fn, CPC mixed with RGD, and CPC grafted with RGD. The degradable microbeads released hUCMSCs at 7 days, which attached to CPC. Adding Fn or RGD to CPC greatly improved cell attachment. CPC grafted with RGD showed the fastest cell proliferation, with cell density being ninefold that on CPC control. The released hUCMSCs underwent osteodifferentiation. Alkaline phosphatase, osteocalcin, collagen 1, and runt-related transcription factor 2 (Runx2) gene expression increased by 10 to 30 fold at 7-21 days, compared with day 1. The released cells on CPC synthesized bone minerals, with the mineralization amount at 21 days being two orders of magnitude higher than that at 7 days. In conclusion, alginate-fibrin microbeads embedded in CPC surface were able to quickly release the hUCMSCs that attached to biofunctionalized CPC. Incorporating Fn and RGD into CPC greatly improved cell function, and CPC grafted with RGD had the fastest cell proliferation. The released cells on CPC differentiated into the osteogenic lineage and synthesized bone minerals. The new biofunctionalized CPC with hUCMSC-encapsulating microbeads is promising for bone regeneration applications.
- Subjects :
- Calcium Phosphates
Scaffold
Alginates
Cell Survival
Biomedical Engineering
Biocompatible Materials
Bioengineering
macromolecular substances
Biochemistry
Fibrin
Umbilical Cord
Biomaterials
chemistry.chemical_compound
Calcification, Physiologic
Glucuronic Acid
Osteogenesis
Materials Testing
Cell Adhesion
Animals
Humans
Cell adhesion
Mechanical Phenomena
Staining and Labeling
biology
Hexuronic Acids
Stem Cells
Mesenchymal stem cell
Bone Cements
technology, industry, and agriculture
Cell Differentiation
Mesenchymal Stem Cells
Original Articles
Microbead (research)
Cells, Immobilized
Glucuronic acid
Microspheres
Fibronectin
chemistry
biology.protein
Cattle
Stem cell
Oligopeptides
Biomedical engineering
Subjects
Details
- ISSN :
- 1937335X and 19373341
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Tissue Engineering Part A
- Accession number :
- edsair.doi.dedup.....bab48fe010ea05a3b7224fdcb75bd0c4
- Full Text :
- https://doi.org/10.1089/ten.tea.2011.0604