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Immunohistochemical localization of telomerase hTERT protein and analysis of clonality in multifocal vulvar intraepithelial neoplasia
- Source :
- American journal of clinical pathology. 114(3)
- Publication Year :
- 2000
-
Abstract
- Vulvar intraepithelial neoplasias (VINs) are potentially premalignant lesions of the squamous mucosa. The immunohistochemical distribution of the catalytic protein subunit of telomerase (hTERT) and the patterns of X chromosome inactivation were investigated as markers of neoplasia in samples from a patient with multifocal and diffuse VIN. hTERT nuclear staining in VIN correlated with squamous maturation and the degree of nuclear atypia. Normal mucosa revealed faint nuclear staining of parabasal cells and lower intermediate layer squamous cells. Monoclonal composition was demonstrated in 0 of 3 samples of VIN1, 2 of 3 samples of VIN2, and 13 of 13 samples of VIN3. The patterns of X chromosome inactivation indicated intramucosal extension and multifocal origin of individual lesions. Five samples of histologically normal vulvar squamous epithelium revealed a random pattern of X chromosome inactivation, consistent with polyclonal composition. All 19 samples from 9 lesions contained human papillomavirus (HPV)-16 sequences. Neither mutations in the p53 tumor suppressor gene or K-ras oncogenes nor loss of heterozygosity at 7 chromosomal loci were detected in any of the 19 samples of VIN. These results demonstrate that HPV-associated VIN may result from multifocal and diffuse 2-dimensional intraepithelial expansion of an immortalized monoclonal cell population.
- Subjects :
- Pathology
medicine.medical_specialty
X Chromosome
Population
Loss of Heterozygosity
Biology
medicine.disease_cause
Polymerase Chain Reaction
Loss of heterozygosity
Catalytic Domain
Dosage Compensation, Genetic
medicine
Humans
Telomerase reverse transcriptase
Nuclear atypia
education
Telomerase
DNA Primers
Intraepithelial neoplasia
education.field_of_study
Vulvar Neoplasms
General Medicine
Middle Aged
medicine.disease
Vulvar intraepithelial neoplasia
Genes, p53
Immunohistochemistry
Clone Cells
DNA-Binding Proteins
Genes, ras
Dysplasia
Mutation
RNA
Female
Carcinogenesis
Carcinoma in Situ
Subjects
Details
- ISSN :
- 00029173
- Volume :
- 114
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- American journal of clinical pathology
- Accession number :
- edsair.doi.dedup.....bada978292824fc8ec6f9db2cf587fa1