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Downregulation of AQP2 expression in the kidney of polydipsic STR/N mice

Authors :
Hiroshi Yamashita
Keiko Tsumura
Kiyotoshi Inenaga
Most.Nahid Parvin
Xuefei Li
Tetsuya Akamatsu
Norio Kanamori
Chenjuan Yao
Kazuo Hosoi
Kwartarini Murdiastuti
Source :
American Journal of Physiology-Renal Physiology. 290:F478-F485
Publication Year :
2006
Publisher :
American Physiological Society, 2006.

Abstract

Aquaporin-2 (AQP2) is responsible for the concentration of urine in the kidney collecting tubule under the regulation of vasopressin. The mRNA level of this water channel in polydipsic STR/N mice was extremely reduced compared with that in normal ICR mice. In male mice, reduction of the AQP2 mRNA level was not evident at 3 wk of age, at which time water intake was not increased. At 10 wk of age, however, the AQP2 mRNA level was reduced to 10% of that in control mice, whereas water intake was increased by 36%. At 44 wk, the water intake became five times that of the control ICR mice, and the AQP2 mRNA level in these polydipsic mice was only ∼5% of control. Similar changes were observed in the AQP2 protein level, suggesting that the mRNA level of AQP2 reflects the protein level of AQP2. These inverse changes in the AQP2 mRNA level and water intake were also evident in female mice. The data imply that polydipsia in STR/N mice may have affected AQP2 mRNA transcription in the kidney, resulting in reduced AQP2 expression, which would contribute to a reduction in overretention of water.

Details

ISSN :
15221466 and 1931857X
Volume :
290
Database :
OpenAIRE
Journal :
American Journal of Physiology-Renal Physiology
Accession number :
edsair.doi.dedup.....baf05ee65b863bcd806d09a5391f7cb4
Full Text :
https://doi.org/10.1152/ajprenal.00029.2005