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High expression of WNT7A predicts poor prognosis and promote tumor metastasis in pancreatic ductal adenocarcinoma
- Source :
- Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
- Publication Year :
- 2018
-
Abstract
- Due to the therapy resistance and frequent metastasis, pancreatic ductal adenocarcinoma(PDAC) remains one of the most malignant carcinoma. WNT7A, an important ligand of Wnt/β-catenin signaling pathways, has a controversial role in tumor development. The role of WNT7A in PDAC remains unclear. In this study, we analyzed the expression pattern of WNT7A at mRNA and protein levels. We found pancreatic cancer tissue demonstrated a significant high WNT7A expression compared with the adjacent non-tumor tissue and the expression of WNT7A positively correlates with poor prognosis and lymph node metastasis. Then, we performed transwell assays and wound healing assays in vitro and found that WNT7A promotes the migration capacity of cancer cells. Furthermore, we explored the underlying mechanism of the WNT7A inducing cell migration. Results showed that up-regulated WNT7A expression inducing higher expression of N-cadherin and lower expression of E-cadherin while the contrast result was shown in the WNT7A knock-down group, which suggested that WNT7A might contribute to an epithelial–mesenchymal transition. Finally, we found that the hypoxia culture condition remarkably increased the WNT7A expression. In conclusion, our work demonstrated that hypoxia induced high expression of WNT7A might promote the cell migration via enhancing the epithelial–mesenchymal transition in PDAC.
- Subjects :
- 0301 basic medicine
Male
Epithelial-Mesenchymal Transition
Pancreatic Ductal Adenocarcinoma (PDAC)
Science
Kaplan-Meier Estimate
Adenocarcinoma
Article
Metastasis
03 medical and health sciences
0302 clinical medicine
Cell Movement
Pancreatic cancer
Cell Line, Tumor
medicine
Carcinoma
Humans
RNA, Messenger
Wnt5a Expression
Neoplasm Metastasis
Wnt Signaling Pathway
Regulation of gene expression
Multidisciplinary
business.industry
Wnt signaling pathway
Cell migration
Middle Aged
medicine.disease
Prognosis
Cell Hypoxia
Up-Regulation
Gene Expression Regulation, Neoplastic
Wnt Proteins
030104 developmental biology
PDAC Patients
030220 oncology & carcinogenesis
Cancer cell
Performed Transwell Assays
Multivariate Analysis
Cancer research
Medicine
Female
Signal transduction
business
PDAC Tissue
Carcinoma, Pancreatic Ductal
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....bb1f28317b7ebe9d0709dc5762856228