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Circular RNAs expression profiles in plasma exosomes from early‐stage lung adenocarcinoma and the potential biomarkers
- Source :
- Journal of Cellular Biochemistry. 121:2525-2533
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- This study aimed to identify differential circular RNA (circRNA) in the plasma exosomes of patients with lung adenocarcinoma (LUAD) using high-throughput sequencing. First, exosomes were isolated using an exosome isolation kit and confirmed by Western blotting, transmission electron microscopy, and NanoSight Assay. Subsequently, plasma circRNA expression profiles were screened by high-throughput sequencing and confirmed by fluorescence quantitative real-time polymerase chain reaction (qRT-PCR) and Sanger sequencing. Finally, the circRNA-miRNA-mRNA network was performed to forecast the potential function of circRNAs. The result of high-throughput sequencing data documented that 182 differentially expressed exosomal circRNAs in all were screened, which included 105 that were upregulated and 78 that were downregulated in LUAD patients plasma compared with controls. The four upregulated circRNAs including circ_0001492, circ_0001346, circ_0000690, and circ_0001439 were identical to the sequencing data by qRT-PCR, and their latent circRNA-miRNA-mRNA interactions were exhibited. Taken together, our study firstly revealed the altered exosomal circRNA expression from plasma samples in patients with LUAD and supports the need for exploring their potential as biomarkers and the pathological effects of lung cancer.
- Subjects :
- Adult
Male
0301 basic medicine
Lung Neoplasms
Adenocarcinoma of Lung
Biology
Exosomes
Biochemistry
Exosome
law.invention
03 medical and health sciences
symbols.namesake
0302 clinical medicine
Circular RNA
law
Biomarkers, Tumor
medicine
Humans
Gene Regulatory Networks
RNA, Messenger
Molecular Biology
Polymerase chain reaction
Aged
Sanger sequencing
RNA, Circular
Cell Biology
Middle Aged
Prognosis
medicine.disease
Molecular biology
Microvesicles
Gene Expression Regulation, Neoplastic
Blot
MicroRNAs
030104 developmental biology
Real-time polymerase chain reaction
Case-Control Studies
030220 oncology & carcinogenesis
symbols
Adenocarcinoma
Female
Subjects
Details
- ISSN :
- 10974644 and 07302312
- Volume :
- 121
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Biochemistry
- Accession number :
- edsair.doi.dedup.....bb430e58f4b7dd9140f82a441fa1cf17