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miR-Let7A Controls the Cell Death and Tight Junction Density of BrainEndothelial Cells under High Glucose Condition
- Source :
- OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, Oxidative Medicine and Cellular Longevity, Oxidative Medicine and Cellular Longevity, Vol 2017 (2017)
- Publication Year :
- 2017
-
Abstract
- Hyperglycemia-induced stress in the brain of patients with diabetes triggers the disruption of blood-brain barrier (BBB), leading to diverse neurological diseases including stroke and dementia. Recently, the role of microRNA becomes an interest in the research for deciphering the mechanism of brain endothelial cell damage under hyperglycemia. Therefore, we investigated whether mircoRNA Let7A (miR-Let7A) controls the damage of brain endothelial (bEnd.3) cells against high glucose condition. Cell viability, cell death marker expressions (p-53, Bax, and cleaved poly ADP-ribose polymerase), the loss of tight junction proteins (ZO-1 and claudin-5), proinflammatory response (interleukin-6, tumor necrosis factor-α), inducible nitric oxide synthase, and nitrite production were confirmed using MTT, reverse transcription-PCR, quantitative-PCR, Western blotting, immunofluorescence, and Griess reagent assay. miR-Let7A overexpression significantly prevented cell death and loss of tight junction proteins and attenuated proinflammatory response and nitrite production in the bEnd.3 cells under high glucose condition. Taken together, we suggest that miR-Let7A may attenuate brain endothelial cell damage by controlling cell death signaling, loss of tight junction proteins, and proinflammatory response against high glucose stress. In the future, the manipulation of miR-Let7A may be a novel solution in controlling BBB disruption which leads to the central nervous system diseases.
- Subjects :
- 0301 basic medicine
Aging
Programmed cell death
Article Subject
Biology
Biochemistry
Cell Line
Tight Junctions
Proinflammatory cytokine
Mice
03 medical and health sciences
0302 clinical medicine
Animals
Viability assay
lcsh:QH573-671
Cell Death
Tight junction
lcsh:Cytology
Brain
Endothelial Cells
Cell Biology
General Medicine
Cell biology
Endothelial stem cell
Nitric oxide synthase
MicroRNAs
Glucose
030104 developmental biology
Cell culture
Hyperglycemia
Immunology
biology.protein
Tumor necrosis factor alpha
030217 neurology & neurosurgery
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, Oxidative Medicine and Cellular Longevity, Oxidative Medicine and Cellular Longevity, Vol 2017 (2017)
- Accession number :
- edsair.doi.dedup.....bb606d5e5ba4b5aca5f24931d2d62810