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Microbiome–Metabolomics Analysis of the Impacts of Long-Term Dietary Advanced-Glycation-End-Product Consumption on C57BL/6 Mouse Fecal Microbiota and Metabolites
- Source :
- Journal of Agricultural and Food Chemistry. 66:8864-8875
- Publication Year :
- 2018
- Publisher :
- American Chemical Society (ACS), 2018.
-
Abstract
- Thermally processed diets are widely consumed, although advanced-glycation end products (AGEs) are unavoidably formed. AGEs, clusters of protein-cross-linking products, become less digestible because they impair intestinal peptidase proteolysis. We characterized the impacts of dietary AGEs on gut microbiota through a microbiome-to-metabolome association study. C57BL/6 mice were fed a heat-treated diet (high-AGE diet, H-AGE) or a standard AIN-93G diet (low-AGE diet, L-AGE) for 8 months. Fecal-microbiota composition was examined by 16S rDNA sequencing, and fecal-metabolome profile was evaluated by gas chromatography-tandem time-of-flight mass spectrometry (GC-TOF-MS). Reduced α-diversity and altered microbiota composition with elevated Helicobacter levels were found in the H-AGE group, and among the 57 perturbed metabolites, protein-fermentation products (i.e., p-cresol and putrescine) were increased. Major dysfunctional metabolic pathways were associated with carbohydrate and amino acid metabolism in two groups. Moreover, high correlations were found between fluctuant gut microbiota and metabolites. These findings might reveal the underlying mechanisms of the detrimental impacts of dietary AGEs on host health.
- Subjects :
- Glycation End Products, Advanced
Male
0301 basic medicine
Gut flora
Gas Chromatography-Mass Spectrometry
Feces
Mice
03 medical and health sciences
chemistry.chemical_compound
Metabolomics
Metabolome
Animals
Microbiome
Food science
Intestinal Mucosa
030109 nutrition & dietetics
Bacteria
biology
General Chemistry
Carbohydrate
biology.organism_classification
Diet
Gastrointestinal Microbiome
Intestines
Mice, Inbred C57BL
Metabolic pathway
030104 developmental biology
chemistry
Putrescine
Advanced glycation end-product
General Agricultural and Biological Sciences
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 66
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....bbbdd698adc811e1bcbc1af8dcde5020