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microRNA-10b promotes the apoptosis of bovine ovarian granulosa cells by targeting plasminogen activator inhibitor-1
- Source :
- Theriogenology. 176:206-216
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Granulosa cells (GCs) are essential somatic cells in the ovaries, and apoptosis of GCs causes follicular atresia. microRNA-10b (miR-10b) is pivotal for cell apoptosis. However, currently, little is known about the role of miR-10b in bovine ovarian GCs (BGCs). In this study, the effect of miR-10b on the apoptosis of BGCs was investigated. Our results showed that the overexpression of miR-10b could increase the apoptosis rate of BGCs, which is associated with the increased expression of Caspase-3 and decreased expression ratio of Bcl-2/Bax (P 0.05). Furthermore, plasminogen activator inhibitor-1 (PAI-1) was confirmed to be a validated target of miR-10b in BGCs using dual-luciferase reporter analysis, and transfection of miR-10b mimics decreased the expression of PAI-1 (P 0.05). In addition, overexpression of PAI-1 significantly inhibited BGC apoptosis (P 0.05), and PAI-1 could alleviate BGC apoptosis induced by miR-10b (P 0.05). Subsequently, phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) was found to be the downstream pathway of PAI-1 by RNA-Seq analysis and verified by Western blot. Finally, a PI3K/AKT inhibitor (Miltefosine) was used to inhibit the PI3K/AKT pathway, which reversed the inhibitory effect of PAI-1 on the apoptosis of BGCs (P 0.05), and enhanced the promotion effect of miR-10b on the apoptosis of BGCs (P 0.05). Our results indicated that miR-10b promotes BGC apoptosis by targeting PAI-1 to regulate the PI3K/AKT pathway.
- Subjects :
- endocrine system
Granulosa Cells
Equine
Somatic cell
Follicular atresia
Follicular Atresia
Apoptosis
MicroRNAs
Phosphatidylinositol 3-Kinases
chemistry.chemical_compound
Microrna 10b
Food Animals
chemistry
Plasminogen activator inhibitor-1
Plasminogen Activator Inhibitor 1
Cancer research
Animals
Cattle
Female
Animal Science and Zoology
Small Animals
Proto-Oncogene Proteins c-akt
Cell Proliferation
Subjects
Details
- ISSN :
- 0093691X
- Volume :
- 176
- Database :
- OpenAIRE
- Journal :
- Theriogenology
- Accession number :
- edsair.doi.dedup.....bc332553e325bfe9bb806a111a605f21