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MicroRNA-124 regulates TGF-α-induced epithelial-mesenchymal transition in human prostate cancer cells
- Source :
- International journal of oncology. 45(3)
- Publication Year :
- 2014
-
Abstract
- Transforming growth factor-α (TGF-α) is upregulated in advanced stages of prostate cancer and strongly correlated with metastasis. However, the effect of TGF-α on epithelial-mesenchymal transition (EMT) in prostate cancer and the underlying mechanisms remain unclear. Recently, microRNAs have emerged as new regulators of EMT. This study found that treatment of DU145 cells with TGF-α suppressed the expression of epithelial marker E-cadherin and increased the expression of mesenchymal marker Vimentin as well as changed the cell morphology from cobblestone shape to spindle shape. The level of miR-124 was downregulated by TGF-α in several different cancer cell lines. Enforced expression of miR-124 abolished TGF-α-induced EMT. Slug was proven to be a target of miR-124 and mediated the inhibitory effect of miR-124 on TGF-α-induced EMT. Furthermore, overexpression of miR-124 reduced the migratory and invasive capacity of TGF-α-treated DU145 cells. In conclusion, our findings suggest that miR-124 inhibits TGF-α-induced EMT in DU145 cells by targeting Slug. Thus, miR-124 may be a potential target for prostate cancer therapeutic intervention.
- Subjects :
- Male
Cancer Research
Pathology
medicine.medical_specialty
Epithelial-Mesenchymal Transition
Biology
Cell morphology
Metastasis
Prostate cancer
DU145
Cancer stem cell
Cell Movement
Cell Line, Tumor
medicine
Humans
Neoplasm Invasiveness
Epithelial–mesenchymal transition
Cancer
Prostatic Neoplasms
Hep G2 Cells
Transforming Growth Factor alpha
medicine.disease
Gene Expression Regulation, Neoplastic
MicroRNAs
Oncology
Cancer cell
Cancer research
Snail Family Transcription Factors
Transcription Factors
Subjects
Details
- ISSN :
- 17912423
- Volume :
- 45
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- International journal of oncology
- Accession number :
- edsair.doi.dedup.....bc668f2487b03a8178091586acc00399