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Development of isotope dilution-liquid chromatography/tandem mass spectrometry for the accurate determination of type-A trichothecenes in grains
- Source :
- Food chemistry. 344
- Publication Year :
- 2020
-
Abstract
- We report the development of a highly accurate method based on isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) for the simultaneous determination of four major type-A trichothecenes in cereal grains. Uniformly labeled [13C] analogues of the target analytes were used as internal standards. An expedient sample preparation procedure was established. After extraction with acetonitrile/water (84:16; v/v), further clean-up was performed using MycoSep 227 solid-phase extraction cartridges. Unlike the commonly used immunoaffinity columns having strong selectivity for only certain target analytes, the cartridges allowed the simultaneous recovery of all four mycotoxins and efficient elimination of co-extracted matrix interferences. The ID-LC-MS/MS method exhibited very good analytical performance in the concentration range of 10–200 µg/kg; accuracy ranged from 97 to 103% with intra-day and inter-day relative standard deviations of less than 5% and 4%, respectively. Measurement uncertainties were generally below 5%. The applicability of the method was assessed by measuring the target mycotoxins in several samples at sub‐µg/kg levels.
- Subjects :
- Analyte
Isotope dilution
Mass spectrometry
01 natural sciences
Zea mays
Trichothecenes, Type A
Analytical Chemistry
Matrix (chemical analysis)
chemistry.chemical_compound
0404 agricultural biotechnology
Liquid chromatography–mass spectrometry
Limit of Detection
Tandem Mass Spectrometry
Sample preparation
Acetonitrile
Chromatography, High Pressure Liquid
Triticum
Carbon Isotopes
Chromatography
Chemistry
010401 analytical chemistry
Extraction (chemistry)
Solid Phase Extraction
Reproducibility of Results
04 agricultural and veterinary sciences
General Medicine
Mycotoxins
040401 food science
0104 chemical sciences
Isotope Labeling
Edible Grain
Food Science
Subjects
Details
- ISSN :
- 18737072
- Volume :
- 344
- Database :
- OpenAIRE
- Journal :
- Food chemistry
- Accession number :
- edsair.doi.dedup.....bc8ddb549f575c581ceb224676d7780c