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New automated and high-throughput quantitative analysis of urinary ketones by multifiber exchange-solid phase microextraction coupled to fast gas chromatography/negative chemical-electron ionization/mass spectrometry
- Source :
- Journal of Automated Methods and Management in Chemistry
- Publication Year :
- 2009
-
Abstract
- The present research is focused on automation, miniaturization, and system interaction with high throughput for multiple and specific Direct Immersion-Solid Phase Microextraction/Fast Gas Chromatography analysis of the urinary ketones. The specific Mass Spectrometry instrumentation, capable of supporting such the automated changeover from Negative Chemical to Electron Ionization mode, as well as the automation of the preparation procedure by new device called MultiFiber Exchange, through change of the fibers, allowed a friendly use of mass spectrometry apparatus with a number of advantages including reduced analyst time and greater reproducibility (2.01–5.32%). The detection limits for the seven ketones were less than 0.004 mg/L. For an innovative powerful meaning in high-throughput routine, the generality of the structurally informative Mass Spectrometry fragmentation patterns together with the chromatographic separation and software automation are also investigated.
- Subjects :
- Detection limit
Reproducibility
Gas-Chromatography
Chromatography
Article Subject
Chemistry
Analytical chemistry
Urinary Analysis
Mass spectrometry
Solid-phase microextraction
Mass Spectrometry
Computer Science Applications
Analytical Chemistry
Fragmentation (mass spectrometry)
Solid phase extraction
Multifiber
Miniaturization
Chemical Engineering (miscellaneous)
Gas chromatography
Urinary Analysi
Instrumentation
Electron ionization
Research Article
Subjects
Details
- ISSN :
- 14645068
- Volume :
- 2010
- Database :
- OpenAIRE
- Journal :
- Journal of automated methodsmanagement in chemistry
- Accession number :
- edsair.doi.dedup.....bca3aebdcefe5545a426a33fc17eacdf