Back to Search
Start Over
A replication study confirms the association of GWAS-identified SNPs at MICB and PLCE1in Thai patients with dengue shock syndrome
- Source :
- BMC Medical Genetics
- Publication Year :
- 2014
- Publisher :
- Springer Science and Business Media LLC, 2014.
-
Abstract
- Background Dengue shock syndrome (DSS), a severe life-threatening form of dengue infection, mostly occurs in children. A recent genome wide association study (GWAS) identified two SNPs, rs3132468 of major histocompatibility complex class I polypeptide-related sequence B (MICB) and rs3765524 of phospholipase C, epsilon 1 (PLCE1), associated with DSS in Vietnamese children. In this study, to examine whether an identical association is found in a different population, the association of these two SNPs with DSS was assessed in Thai children with dengue. Methods The rs3132468 and rs3765524 SNPs were genotyped in 917 Thai children with dengue: 76 patients with DSS and 841 patients with non-DSS. The allele frequencies were compared between DSS and non-DSS groups by one-sided Fisher’s exact test. The association of rs3132468 and rs3765524 with the mRNA expression levels of MICB and PLCE1 were assessed in EBV-transformed lymphoblastoid cell lines. Results The reported DSS-risk alleles were significantly associated with DSS in Thai patients with dengue (one-sided P = 0.0213 and odds ratio [OR] = 1.58 for rs3132468-C and one-sided P = 0.0252 and OR = 1.49 for rs3765524-C). The rs3132468-C allele showed a significant association with lower mRNA level of MICB (P = 0.0267), whereas the rs3765524-C allele did not. These results imply that the MICB molecule may play an important role in the prevention of DSS in dengue infection. Conclusions Together with previous association studies, we conclude that rs3132468-C at MICB and rs3765524-C at PLCE1 confer risk of DSS in Southeast Asians.
- Subjects :
- Male
Adolescent
Genotype
Population
Gene Expression
Expression
Single-nucleotide polymorphism
Genome-wide association study
Biology
Polymorphism, Single Nucleotide
Dengue fever
Association
Phosphoinositide Phospholipase C
Asian People
Gene Frequency
PLCE1
Genetics
medicine
Humans
Genetic Predisposition to Disease
Genetics(clinical)
RNA, Messenger
Severe Dengue
Polymorphism
Allele
Child
education
Allele frequency
Alleles
Genetics (clinical)
Dengue shock syndrome (DSS)
Genetic association
education.field_of_study
Histocompatibility Antigens Class I
Infant, Newborn
Computational Biology
Infant
Odds ratio
Thailand
medicine.disease
digestive system diseases
stomatognathic diseases
MICB
Child, Preschool
Immunology
Female
Genome-Wide Association Study
Research Article
Subjects
Details
- ISSN :
- 14712350
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- BMC Medical Genetics
- Accession number :
- edsair.doi.dedup.....bcb72de1c597987a02b17c73bf26e521