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Integrated Analysis of miR-430 on Steroidogenesis-Related Gene Expression of Larval Rice Field Eel Monopterus albus
- Source :
- International Journal of Molecular Sciences, Vol 22, Iss 6994, p 6994 (2021), International Journal of Molecular Sciences, Volume 22, Issue 13
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- The present study aims to reveal the mechanism by which miR-430s regulate steroidogenesis in larval rice field eel Monopterus albus. To this end, M. albus embryos were respectively microinjected with miRNA-overexpressing mimics (agomir430a, agomir430b, and agomir430c) or miRNA-knockdown inhibitors (antagomir430a, antagomir430b, and antagomir430c). Transcriptome profiling of the larvae indicated that a total of more than 149 differentially expressed genes (DEGs) were identified among the eight treatments. Specifically, DEGs related to steroidogenesis, the GnRH signaling pathway, the erbB signaling pathway, the Wnt signaling pathway, and other pathways were characterized in the transcriptome. We found that steroidogenesis-related genes (hydroxysteroid 17-beta dehydrogenase 3 (17β-hsdb3), hydroxysteroid 17-beta dehydrogenase 7 (17β-hsdb7), hydroxysteroid 17-beta dehydrogenase 12 (17β-hsdb12), and cytochrome P450 family 19 subfamily a (cyp19a1b)) were significantly downregulated in miR-430 knockdown groups. The differential expressions of miR-430 in three gonads indicated different roles of three miR-430 (a, b, and c) isoforms in regulating steroidogenesis and sex differentiation. Mutation of the miR-430 sites reversed the downregulation of cytochrome P450 family 17 (cyp17), cyp19a1b, and forkhead box L2 (foxl2) reporter activities by miR-430, indicating that miR-430 directly interacted with cyp17, cyp19a1b, and foxl2 genes to inhibit their expressions. Combining these findings, we concluded that miR-430 regulated the steroidogenesis and the biosynthesis of steroid hormones by targeting cyp19a1b in larval M. albus. Our results provide a novel insight into steroidogenesis at the early stage of fish at the molecular level.
- Subjects :
- 0301 basic medicine
steroidogenesis
ERBB signaling pathway
QH301-705.5
030209 endocrinology & metabolism
Biology
Monopterus albus
Catalysis
Article
miR-430
Inorganic Chemistry
Transcriptome
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Downregulation and upregulation
Animals
Physical and Theoretical Chemistry
Biology (General)
Molecular Biology
3' Untranslated Regions
QD1-999
Spectroscopy
Gene knockdown
Sexual differentiation
Gene Expression Profiling
Organic Chemistry
Wnt signaling pathway
Fishes
Computational Biology
General Medicine
early stage
Computer Science Applications
Cell biology
Biosynthetic Pathways
MicroRNAs
Chemistry
030104 developmental biology
Forkhead box L2
chemistry
Gene Expression Regulation
Multigene Family
Steroids
Hydroxysteroid
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Volume :
- 22
- Issue :
- 6994
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....bccbcd6d6a1b0bc33dfe0e5bc35f156d