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An antimicrobial peptide-resistant minor subpopulation of Photorhabdus luminescens is responsible for virulence
- Source :
- Scientific Reports, Scientific Reports, Nature Publishing Group, 2017, 7, 〈10.1038/srep43670〉, Scientific Reports, Nature Publishing Group, 2017, 7, pp.43670. ⟨10.1038/srep43670⟩, Scientific Reports, 2017, 7, pp.43670. ⟨10.1038/srep43670⟩, Scientific Reports (7), 43670. (2017)
- Publication Year :
- 2017
- Publisher :
- Nature Publishing Group, 2017.
-
Abstract
- Some of the bacterial cells in isogenic populations behave differently from others. We describe here how a new type of phenotypic heterogeneity relating to resistance to cationic antimicrobial peptides (CAMPs) is determinant for the pathogenic infection process of the entomopathogenic bacterium Photorhabdus luminescens. We demonstrate that the resistant subpopulation, which accounts for only 0.5% of the wild-type population, causes septicemia in insects. Bacterial heterogeneity is driven by the PhoPQ two-component regulatory system and expression of pbgPE, an operon encoding proteins involved in lipopolysaccharide (LPS) modifications. We also report the characterization of a core regulon controlled by the DNA-binding PhoP protein, which governs virulence in P. luminescens. Comparative RNAseq analysis revealed an upregulation of marker genes for resistance, virulence and bacterial antagonism in the pre-existing resistant subpopulation, suggesting a greater ability to infect insect prey and to survive in cadavers. Finally, we suggest that the infection process of P. luminescens is based on a bet-hedging strategy to cope with the diverse environmental conditions experienced during the lifecycle.
- Subjects :
- 0301 basic medicine
Insecta
Operon
[SDV]Life Sciences [q-bio]
[ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition
030106 microbiology
Population
salmonella
Virulence
Drug resistance
[ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
peptide antimicrobien
Article
Bacterial genetics
Microbiology
03 medical and health sciences
inhomogeneity
Disk Diffusion Antimicrobial Tests
Photorhabdus luminescens
Drug Resistance, Bacterial
Gene Order
hétérogénéité
Animals
education
ComputingMilieux_MISCELLANEOUS
photorhabdus luminescens
bactérie
education.field_of_study
Multidisciplinary
biology
Gene Expression Profiling
Enterobacteriaceae Infections
biology.organism_classification
bacterium
030104 developmental biology
Regulon
Gene Expression Regulation
Genes, Bacterial
Mutation
Photorhabdus
Antimicrobial Cationic Peptides
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....bcce8e3df37be126eb3e87fdf6f9a20a
- Full Text :
- https://doi.org/10.1038/srep43670