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Modulation of translation factor's gene expression by histone deacetylase inhibitors in breast cancer cells
- Source :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Publication Year :
- 2005
- Publisher :
- De Gruyter, 2005.
-
Abstract
- The histone deacetylase inhibitors sodium butyrate (NaBu) and trichostatin A (TSA) exhibit anti-proliferative activity by causing cell cycle arrest and apoptosis. The mechanisms by which NaBu and TSA cause apoptosis and cell cycle arrest are not yet completely clarified, although these agents are known to modulate the expression of several genes including cell-cycle- and apoptosis-related genes. The enzymes involved in the process of translation have important roles in controlling cell growth and apoptosis, and several of these translation factors have been described as having a causal role in the development of cancer. The expression patterns of the translation mechanism, namely of the elongation factors eEF1A1 and eEF1A2, and of the termination factors eRF1 and eRF3, were studied in the breast cancer cell line MCF-7 by real-time quantitative reverse transcription-polymerase chain reaction after a 24-h treatment with NaBu and TSA. NaBu induced inhibition of translation factors' transcription, whereas TSA caused an increase in mRNA levels. Thus, these two agents may modulate the expression of translation factors through different pathways. We propose that the inhibition caused by NaBu may, in part, be responsible for the cell cycle arrest and apoptosis induced by this agent in MCF-7 cells.
- Subjects :
- Clinical Biochemistry
Peptide Chain Elongation, Translational
Antineoplastic Agents
Breast Neoplasms
Apoptosis
Biology
Cell cycle
Hydroxamic Acids
Histone Deacetylases
chemistry.chemical_compound
Histone deacetylase inhibitors
Antineoplastic agents
medicine
Tumor Cells, Cultured
Humans
Translation factor
Enzyme Inhibitors
Pharmacology
Gene expression regulation
Peptide chain elongation, Translational
Cell growth
Tumor cells, Cultured
Biochemistry (medical)
Cell Cycle
Sodium butyrate
Enzyme inhibitors
General Medicine
Molecular biology
Eukaryotic translation elongation factor 1 alpha 1
Genetic translation
Hydroxamic acids
Protein biosynthesis
Cell biology
Gene Expression Regulation, Neoplastic
Histone Deacetylase Inhibitors
Medical Laboratory Technology
Butyrates
Trichostatin A
chemistry
Protein Biosynthesis
Female
Histone deacetylase
Breast neoplasms
medicine.drug
Genes, Neoplasm
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Accession number :
- edsair.doi.dedup.....bcd98e9fc666152d5f37e66fe8fb3baa