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Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions
- Source :
- Journal of cell science. 128(6)
- Publication Year :
- 2015
-
Abstract
- Embryonic development and adult tissue homeostasis require precise information exchange between cells and their microenvironment to coordinate cell behavior. A specialized class of ultra-long actin-rich filopodia, termed cytonemes, provides one mechanism for this spatiotemporal regulation of extracellular cues. We provide here a mechanism whereby the stem-cell marker Lgr5, and its family member Lgr4, promote the formation of cytonemes. Lgr4- and Lgr5-induced cytonemes exceed lengths of 80 µm, are generated through stabilization of nascent filopodia from an underlying lamellipodial-like network and functionally provide a pipeline for the transit of signaling effectors. As proof-of-principle, we demonstrate that Lgr5-induced cytonemes act as conduits for cell signaling by demonstrating that the actin motor and filopodial cargo carrier protein myosin X (Myo10) and the G-protein-coupled receptor (GPCR) signaling effector β-arrestin-2 (Arrb2) transit into cytonemes. This work delineates a biological function for Lgr4 and Lgr5 and provides the rationale to fully investigate Lgr4 and Lgr5 function and cytonemes in mammalian stem cell and cancer stem cell behavior.
- Subjects :
- Adult
Cell signaling
Arrestins
Blotting, Western
Cell Surface Extension
Biology
Receptors, G-Protein-Coupled
Cell membrane
Cancer stem cell
Myosin
medicine
Humans
Immunoprecipitation
Pseudopodia
Molecular Biology
Actin
Tissue homeostasis
beta-Arrestins
Beta-Arrestins
Stem Cells
Cell Membrane
LGR5
Biological Transport
Cell Biology
beta-Arrestin 2
Actins
Cell biology
medicine.anatomical_structure
HEK293 Cells
Cell Surface Extensions
Filopodia
Developmental Biology
Cytoneme
Signal Transduction
Research Article
Subjects
Details
- ISSN :
- 14779137
- Volume :
- 128
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of cell science
- Accession number :
- edsair.doi.dedup.....bce72bd98d07007fd054c13261657fbd