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Elevated urinary excretion of free pyridinoline in Friesian horses suggests a breed-specific increase in collagen degradation
- Source :
- BMC Veterinary Research, Vol 14, Iss 1, Pp 1-7 (2018), BMC VETERINARY RESEARCH, BMC Veterinary Research
- Publication Year :
- 2018
- Publisher :
- BMC, 2018.
-
Abstract
- Background: Friesian horses are known for their high inbreeding rate resulting in several genetic diseases such as hydrocephaly and dwarfism. This last decade, several studies focused on two other presumed hereditary traits in Friesian horses: megaoesophagus and aortic rupture. The pathogenesis of these diseases remains obscure but an important role of collagen has been hypothesized. The purpose of this study was to examine possible breed-related differences in collagen catabolism. Urinary specimens from Friesian (n = 17, median age 10 years old) and Warmblood horses (n = 17, median age 10 years old) were assessed for mature collagen cross-links, i.e. pyridinoline (PYD) (= hydroxylysylpyridinoline/HP) and deoxypyridinoline (DPD) (lysylpyridinoline /LP). Solid-phase extraction was performed, followed by reversed-phase ion-paired liquid chromatography prior to tandem mass spectrometry (MS/MS) detection. Results: Mean urinary concentrations of free PYD, expressed as fPYD/creatinine ratio, were significantly higher in Friesian horses compared to Warmblood horses (28.5 +/- 5.2 versus 22.2 +/- 9.6 nmol/mmol, p = 0.02) while mean fDPD/creatinine ratios were similar in both horse breeds (3.0 +/- 0.7 versus 4.6 +/- 3.7 nmol/mmol, p = 0.09). Conclusions: Since DPD is considered a specific bone degradation marker and PYD is more widely distributed in connective tissues, the significant elevation in the mean PYD/DPD ratio in Friesian versus Warmblood horses (9.6 +/- 1.6 versus 5.7 +/- 1.8, p < 0.0001) suggests a soft tissue origin for the increased fPYD levels. Considering that a previous study found no differences in total collagen content between Friesian and Warmblood horses for tendon and aortic tissue, this indicates a higher rate of collagen degradation. The latter might, at least in part, explain the predisposition of Friesians to connective tissue disorders.
- Subjects :
- 0301 basic medicine
Male
Deoxypyridinoline
Dwarfism
AORTIC RUPTURE
Horse
0403 veterinary science
chemistry.chemical_compound
MARKERS
Inbreeding
Amino Acids
Pyridinoline
lcsh:Veterinary medicine
04 agricultural and veterinary sciences
General Medicine
Aortic rupture
Breed
medicine.anatomical_structure
Warmblood
PREGNANCY
DISEASES
Female
TURNOVER
Collagen
Research Article
medicine.medical_specialty
040301 veterinary sciences
Connective tissue
03 medical and health sciences
Cross-links
AGE
Species Specificity
BONE-RESORPTION
Internal medicine
medicine
Animals
Horses
Veterinary Sciences
ELASTIN
Creatinine
General Veterinary
Mass spectrometry
business.industry
Collagen Diseases
PYRIDINIUM CROSSLINKS
medicine.disease
030104 developmental biology
Endocrinology
chemistry
lcsh:SF600-1100
Horse Diseases
INVOLUTION
Megaoesophagus
business
Subjects
Details
- Language :
- English
- ISSN :
- 17466148
- Volume :
- 14
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Veterinary Research
- Accession number :
- edsair.doi.dedup.....bd45dd6351f711902ed13c7e18c24a2c