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Proteomic Characterization of Host-Pathogen Interactions during Bovine Trophoblast Cell Line Infection by Neospora caninum
- Source :
- Pathogens, Vol 9, Iss 749, p 749 (2020), Pathogens, Volume 9, Issue 9, PATHOGENS
- Publication Year :
- 2020
-
Abstract
- Despite the importance of bovine neosporosis, relevant knowledge gaps remain concerning the pathogenic mechanisms of Neospora caninum. Infection of the placenta is a crucial event in the pathogenesis of the disease<br />however, very little is known about the relation of the parasite with this target organ. Recent studies have shown that isolates with important variations in virulence also show different interactions with the bovine trophoblast cell line F3 in terms of proliferative capacity and transcriptome host cell modulation. Herein, we used the same model of infection to study the interaction of Neospora with these target cells at the proteomic level using LC-MS/MS over the course of the parasite lytic cycle. We also analysed the proteome differences between high- (Nc-Spain7) and low-virulence (Nc-Spain1H) isolates. The results showed that mitochondrial processes and metabolism were the main points of Neospora-host interactions. Interestingly, Nc-Spain1H infection showed a higher level of influence on the host cell proteome than Nc-Spain7 infection.
- Subjects :
- Microbiology (medical)
proteome
Neospora caninum
lcsh:Medicine
Virulence
Q1
Article
Microbiology
Transcriptome
Neospora
parasitic diseases
Immunology and Allergy
Parasite hosting
Molecular Biology
Pathogen
General Immunology and Microbiology
biology
bovine trophoblast cell line
host–parasite interaction
lcsh:R
R735
biology.organism_classification
QR
Infectious Diseases
Lytic cycle
Proteome
isolate virulence
Subjects
Details
- Language :
- English
- ISSN :
- 20760817
- Database :
- OpenAIRE
- Journal :
- Pathogens, Vol 9, Iss 749, p 749 (2020), Pathogens, Volume 9, Issue 9, PATHOGENS
- Accession number :
- edsair.doi.dedup.....bdcd59ffe815467e6117c53eb7960556