Back to Search
Start Over
Human T‐cell Leukemia Virus Type 1 Can Infect a Wide Variety of Cells in Mice
- Source :
- Japanese Journal of Cancer Research : Gann
- Publication Year :
- 1999
- Publisher :
- Blackwell Publishing Ltd, 1999.
-
Abstract
- Analysis of human T-cell leukemia virus type 1 (HTLV-1)-infected cell types and the interplay of these infected cells in vivo should provide valuable information to elucidate the pathogenesis of HTLV-1-associated diseases in humans and in animal models. In this study, HTLV-1-infected cell types were identified in HTLV-1-infected C3H/HeJ mice. Pan T, CD 4 + , CD 8 + , granulocyte and pan B cell fractions in the splenocytes of MT-2 cell-inoculated mice were sorted by use of their cell surface high-density expression of CD CD 4 , CD 8 , Gr-1 and B 220 antigens, respectively, with a fluorescence-activated cell sorter. The pX sequence of HTLV-1 provirus in the lysate of each fraction was amplified by polymerase chain reaction and detected by Southern hybridization. Interestingly, in addition to the CD 4 + cell fraction, the pX sequence was also found in CD 8 + cell, B cell and granulocyte fractions. The hroad cell spectrum of HTLV-1 infection in mice is consistent with the situation in humans. Our findings indicate that HTLV-1 receptor or coreceptor is widely distributed among different cell types in mice.
- Subjects :
- CD4-Positive T-Lymphocytes
Cancer Research
Cell type
Cellular tropism
Mouse
viruses
T-Lymphocytes
Cell
Biology
CD8-Positive T-Lymphocytes
Virus
Article
Cell Line
Mice
Antigen
medicine
Animals
Humans
Receptor
HTLV‐
B cell
B-Lymphocytes
Human T-lymphotropic virus 1
Mice, Inbred C3H
Provirus
medicine.disease
Flow Cytometry
Virology
Antigens, Differentiation
HTLV-I Infections
Leukemia
medicine.anatomical_structure
Oncology
Receptors, Virus
Spleen
Granulocytes
Subjects
Details
- Language :
- English
- ISSN :
- 18764673 and 09105050
- Volume :
- 90
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Japanese Journal of Cancer Research : Gann
- Accession number :
- edsair.doi.dedup.....bddb3da4946cce54ba53f7fb1d198f54