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Effects of glucose metabolism during in vitro maturation on cytoplasmic maturation of mouse oocytes
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Although there are many reports on the effect of glucose metabolism on oocyte nuclear maturation, there are few studies on its effect on ooplasmic maturation. By manipulating glucose metabolism pathways using a maturation medium that could support oocyte nuclear maturation but only a limited blastocyst formation without glucose, this study determined effects of glucose metabolism pathways on ooplasmic maturation. During maturation of cumulus-oocyte-complexes (COCs) with glucose, the presence of PPP inhibitor, DHEA or glycolysis inhibitor, iodoacetate significantly decreased blastocyst rates, intraoocyte glutathione and ATP. While blastocyst rates, GSH/GSSG ratio and NADPH were higher, ROS was lower significantly in COCs matured with iodoacetate than with DHEA. Fructose-6-phosphate overcame the inhibitory effect of DHEA on PPP. During maturation of COCs with pyruvate, electron transport inhibitor, rotenone or monocarboxylate transfer inhibitor, 4-CIN significantly decreased blastocyst rates. Cumulus-denuded oocytes had a limited capacity to use glucose or lactate, but they could use pyruvate to support maturation. In conclusion, whereas glycolysis promoted ooplasmic maturation mainly by supplying energy, PPP facilitated ooplasmic maturation to a greater extent by both reducing oxidative stress and supplying energy through providing fructose-6-phosphate for glycolysis. Pyruvate was transferred by monocarboxylate transporters and utilized through mitochondrial electron transport to sustain ooplasmic maturation.
- Subjects :
- 0301 basic medicine
Cytoplasm
medicine.medical_specialty
Carbohydrate metabolism
Biology
medicine.disease_cause
Article
Andrology
Mice
03 medical and health sciences
chemistry.chemical_compound
Internal medicine
medicine
Animals
Glycolysis
Blastocyst
Cells, Cultured
Cumulus Cells
Multidisciplinary
Rotenone
Glutathione
Oocyte
Coculture Techniques
In vitro maturation
Glucose
030104 developmental biology
medicine.anatomical_structure
Endocrinology
chemistry
Oocytes
Female
Oxidative stress
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....bdeca2e383ce60cc5843217067bce5b0
- Full Text :
- https://doi.org/10.1038/srep20764