Procion dyes as matrix markers in growing bone and teeth

Twelve mono- and dichloro-s-triazinyl dyes were selected to ascertain the vital-staining characteristics of these dyes, to evaluate the toxicity, and to establish an optimal rate of doseage for use with several animal species. In addition to these dyes (also known in the dye industry as Procion compounds), tetracycline hydrochloride and a hematoporphyrin derivative were also studied. the experimental animals used were mice, rats, rabbits, cats, dogs, and monkeys. The agents were given in a single dose or multiple doses at selected time intervals, after which the experimental animals were sacrificed and the tissue removed for examination. The tissues were examined grossly and with various microscopic methods, and after being placed in clearing solutions for various time periods. The dyes selectively stained bone, dentin, and cementum, which were being formed at the time of administration. Certain dyes have marked advantages over others, not only in staining characteristics but also in toxicity and fluorescence.