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Identification of mature appressorium-enriched transcripts in Magnaporthe grisea, the rice blast fungus, using suppression subtractive hybridization
- Source :
- FEMS microbiology letters. 245(1)
- Publication Year :
- 2004
-
Abstract
- We have constructed a fungal subtractive suppressive library enriched in genes expressed during appressorium maturation in Magnaporthe grisea. Sequencing of 250 clones from the subtracted appressorium cDNA library revealed 142 unique genes, represented by 155 non-redundant ESTs (expressed sequence tags). Of these ESTs, 72 have not been previously isolated in M. grisea. RT-PCR analysis of 105 of the genes discovered found transcripts corresponding to 71 of the ESTs only in mature appressoria while transcripts corresponding to a further 34 of the isolated ESTs were expressed both in appressoria and conidia/mycelia. Genes specifically expressed in appressorium identified by SSH included a number that have been previously implicated in appressorium formation or function including GAS1, GAS3, and PTH11.
- Subjects :
- Transcription, Genetic
Fungus
Microbiology
Fungal Proteins
Complementary DNA
Gene Expression Regulation, Fungal
Genetics
Magnaporthe grisea
Molecular Biology
Gene
Gene Library
Expressed Sequence Tags
Appressorium
Expressed sequence tag
biology
cDNA library
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
fungi
food and beverages
Nucleic Acid Hybridization
Oryza
Sequence Analysis, DNA
biology.organism_classification
Molecular biology
Magnaporthe
Suppression subtractive hybridization
Subjects
Details
- ISSN :
- 03781097
- Volume :
- 245
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- FEMS microbiology letters
- Accession number :
- edsair.doi.dedup.....be651e0d0d2aa6d7b548bd99b236d4a2