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Development and Characterization of Protective Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin and Comparison with Other Subunit and Commercial Vaccines
- Source :
- Clinical and Vaccine Immunology. 18:50-58
- Publication Year :
- 2011
- Publisher :
- American Society for Microbiology, 2011.
-
Abstract
- Haemophilus parasuis is the agent responsible for causing Glässer's disease, which is characterized by fibrinous polyserositis, polyarthritis, and meningitis in pigs. In this study, we have characterized native outer membrane proteins with affinity to porcine transferrin (NPAPT) from H. parasuis serovar 5, Nagasaki strain. This pool of proteins was used as antigen to developed two vaccine formulations: one was adjuvanted with a mineral oil (Montanide IMS 2215 VG PR), while the other was potentiated with a bacterial neuraminidase from Clostridium perfringens . The potential protective effect conferred by these two vaccines was compared to that afforded by two other vaccines, consisting of recombinant transferrin-binding protein (rTbp) A or B fragments from H. parasuis , Nagasaki strain, and by a commercially available inactivated vaccine. Five groups of colostrum-deprived piglets immunized with the vaccines described above, one group per each vaccine, and a group of nonvaccinated control animals were challenged intratracheally with a lethal dose (3 × 10 8 CFU) of H. parasuis , Nagasaki strain. The two vaccines containing rTbps yielded similar results with minimal protection against death, clinical signs, gross and microscopic lesions, and H. parasuis invasion. In contrast, the two vaccines composed of NPAPT antigen and commercial bacterin resulted in a strong protection against challenge (without deaths and clinical signs), mild histopathological changes, and no recovery of H. parasuis , thus suggesting their effectiveness in preventing Glässer's disease outbreaks caused by serovar 5.
- Subjects :
- Microbiology (medical)
Serotype
Haemophilus Infections
Swine
Clinical Biochemistry
Immunology
Biology
medicine.disease_cause
Microbiology
Haemophilus parasuis
Antigen
Immunity
Haemophilus
medicine
Animals
Immunology and Allergy
Haemophilus Vaccines
Swine Diseases
Transferrin
Clostridium perfringens
Vaccine Research
biology.organism_classification
Virology
Recombinant Proteins
Transferrin-Binding Protein A
Survival Rate
Vaccination
Vaccines, Inactivated
Vaccines, Subunit
Inactivated vaccine
biology.protein
Immunization
Neuraminidase
Bacterial Outer Membrane Proteins
Subjects
Details
- ISSN :
- 1556679X and 15566811
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Clinical and Vaccine Immunology
- Accession number :
- edsair.doi.dedup.....be9b84926fce1f69c2a9905ddbc2c47f