Back to Search
Start Over
Transcriptome-wide analysis links the short-term expression of the b isoforms of TIA proteins to protective proteostasis-mediated cell quiescence response
- Source :
- PLoS ONE, PLoS ONE, Vol 13, Iss 12, p e0208526 (2018), Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2018
-
Abstract
- Control of gene expression depends on genetics and environmental factors. The T-cell intracellular antigens T-cell intracellular antigen 1 (TIA1), TIA1-like/related protein (TIAL1/TIAR) and human antigen R (HuR/ELAVL1) are RNA-binding proteins that play crucial roles in regulating gene expression in both situations. This study used massive sequencing analysis to uncover molecular and functional mechanisms resulting from the short-time expression of the b isoforms of TIA1 and TIAR, and of HuR in HEK293 cells. Our gene profiling analysis identified several hundred differentially expressed genes (DEGs) and tens of alternative splicing events associated with TIA1b, TIARb and HuR overexpression. Gene ontology analysis revealed that the controlled expression of these proteins strongly influences the patterns of DEGs and RNA variants preferentially associated with development, reproduction, cell cycle, metabolism, autophagy and apoptosis. Mechanistically, TIA1b and TIARb isoforms display both common and differential effects on the regulation of gene expression, involving systematic perturbations of cell biosynthetic machineries (splicing and translation). The transcriptome outputs were validated using functional assays of the targeted cellular processes as well as expression analysis for selected genes. Collectively, our observations suggest that early TIA1b and TIARb expression operates to connect the regulatory crossroads to protective proteostasis responses associated with a survival quiescence phenotype.<br />Spanish Ministry of Economic Affairs and Competitiveness to JMI (BFU2014-57735R). The CBMSO receives institutional funding from Fundación Ramón Areces and Banco Santander
- Subjects :
- 0301 basic medicine
Gene Expression
Biochemistry
ELAV-Like Protein 1
Transcriptome
Gene expression
Protein Isoforms
Cell Cycle and Cell Division
Protein Metabolism
Regulation of gene expression
Multidisciplinary
Gene Ontologies
Messenger RNA
RNA-Binding Proteins
Genomics
Cell biology
Nucleic acids
Cell Processes
Medicine
Research Article
Cell Physiology
TIA1
Science
Biology
03 medical and health sciences
Cell quiescence
Genetics
Humans
Cell Proliferation
Gene Expression Profiling
Alternative splicing
Biology and Life Sciences
Computational Biology
Cell Biology
Genome Analysis
G1 Phase Cell Cycle Checkpoints
T-Cell Intracellular Antigen-1
Cell Metabolism
Gene expression profiling
Alternative Splicing
030104 developmental biology
Proteostasis
Gene Ontology
HEK293 Cells
Metabolism
RNA processing
RNA
Protein Translation
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....beb1dee719491a209adac4c4f4215126