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A copper(<scp>ii</scp>)–dipicolylamine–coumarin sensor for maltosyltransferase assay
- Source :
- Dalton Transactions. 48:8026-8029
- Publication Year :
- 2019
- Publisher :
- Royal Society of Chemistry (RSC), 2019.
-
Abstract
- A Cu(ii)-[di(2-methylpyridyl)methylamino]coumarin fluorescence turn-on sensor (Cu-1b) is designed to detect phosphate ions with Kass = 1.4 × 105 M-1 in HEPES buffer. Cu-1b is applied to probe the GlgE-catalyzed maltose-transfer reaction of α-maltose-1-phosphate to α-1,4-glucan with concomitant release of phosphate ions in Mycobacterium tuberculosis.
- Subjects :
- chemistry.chemical_element
010402 general chemistry
01 natural sciences
Inorganic Chemistry
Hepes buffer
chemistry.chemical_compound
Coumarins
Organometallic Compounds
Amines
Picolinic Acids
Enzyme Assays
Maltosyltransferase
010405 organic chemistry
Chemistry
Coumarin
Phosphate
Fluorescence
Copper
0104 chemical sciences
Dipicolylamine
Glucosyltransferases
Biocatalysis
Dimerization
Nuclear chemistry
Subjects
Details
- ISSN :
- 14779234 and 14779226
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Dalton Transactions
- Accession number :
- edsair.doi.dedup.....bebb6734b86802ffc560a242ae8fd177