Back to Search
Start Over
Specific uptake and genotoxicity induced by polystyrene nanobeads with distinct surface chemistry on human lung epithelial cells and macrophages
- Source :
- PLoS ONE, PLoS ONE, Public Library of Science, 2015, 10 (4), pp.art. e0123297. ⟨10.1371/journal.pone.0123297⟩, PLoS ONE, 2015, 10 (4), pp.art. e0123297. ⟨10.1371/journal.pone.0123297⟩, PLoS ONE, Vol 10, Iss 4, p e0123297 (2015)
- Publication Year :
- 2015
- Publisher :
- HAL CCSD, 2015.
-
Abstract
- Nanoparticle surface chemistry is known to play a crucial role in interactions with cells and their related cytotoxic effects. As inhalation is a major route of exposure to nanoparticles, we studied specific uptake and damages of well-characterized fluorescent 50 nm polystyrene (PS) nanobeads harboring different functionalized surfaces (non-functionalized, carboxylated and aminated) on pulmonary epithelial cells and macrophages (Calu-3 and THP-1 cell lines respectively). Cytotoxicity of in mass dye-labeled functionalized PS nanobeads was assessed by xCELLigence system and alamarBlue viability assay. Nanobeads-cells interactions were studied by video-microscopy, flow cytometry and also confocal microscopy. Finally ROS generation was assessed by glutathione depletion dosages and genotoxicity was assessed by γ-H2Ax foci detection, which is considered as the most sensitive technique for studying DNA double strand breaks. The uptake kinetic was different for each cell line. All nanobeads were partly adsorbed and internalized, then released by Calu-3 cells, while THP-1 macrophages quickly incorporated all nanobeads which were located in the cytoplasm rather than in the nuclei. In parallel, the genotoxicity study reported that only aminated nanobeads significantly increased DNA damages in association with a strong depletion of reduced glutathione in both cell lines. We showed that for similar nanoparticle concentrations and sizes, aminated polystyrene nanobeads were more cytotoxic and genotoxic than unmodified and carboxylated ones on both cell lines. Interestingly, aminated polystyrene nanobeads induced similar cytotoxic and genotoxic effects on Calu-3 epithelial cells and THP-1 macrophages, for all levels of intracellular nanoparticles tested. Our results strongly support the primordial role of nanoparticles surface chemistry on cellular uptake and related biological effects. Moreover our data clearly show that nanoparticle internalization and observed adverse effects are not necessarily associated.
- Subjects :
- Cell Survival
Surface Properties
lcsh:Medicine
02 engineering and technology
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Flow cytometry
Cell Line
Histones
Oxazines
medicine
Fluorescence microscope
Cytotoxic T cell
Humans
DNA Breaks, Double-Stranded
Viability assay
Particle Size
lcsh:Science
Cytotoxicity
0105 earth and related environmental sciences
Amination
Multidisciplinary
medicine.diagnostic_test
Chemistry
Macrophages
lcsh:R
Epithelial Cells
021001 nanoscience & nanotechnology
Molecular biology
Glutathione
3. Good health
Xanthenes
Cell culture
[SDV.TOX]Life Sciences [q-bio]/Toxicology
[SDE]Environmental Sciences
Biophysics
Nanoparticles
Polystyrenes
lcsh:Q
0210 nano-technology
Reactive Oxygen Species
Intracellular
Genotoxicity
Research Article
DNA Damage
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Public Library of Science, 2015, 10 (4), pp.art. e0123297. ⟨10.1371/journal.pone.0123297⟩, PLoS ONE, 2015, 10 (4), pp.art. e0123297. ⟨10.1371/journal.pone.0123297⟩, PLoS ONE, Vol 10, Iss 4, p e0123297 (2015)
- Accession number :
- edsair.doi.dedup.....bed2ae64cd02bac94d652f30de5eef54