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Systems biology of the IMIDIA biobank from organ donors and pancreatectomised patients defines a novel transcriptomic signature of islets from individuals with type 2 diabetes
- Source :
- Diabetologia 61, 641–657 (2018), Diabetologia, Diabetologia, vol. 61, no. 3, pp. 641-657, Diabetologia, Vol. 61, No 3 (2018) pp. 641-657
- Publication Year :
- 2018
- Publisher :
- Springer, 2018.
-
Abstract
- Aims/hypothesis Pancreatic islet beta cell failure causes type 2 diabetes in humans. To identify transcriptomic changes in type 2 diabetic islets, the Innovative Medicines Initiative for Diabetes: Improving beta-cell function and identification of diagnostic biomarkers for treatment monitoring in Diabetes (IMIDIA) consortium (www.imidia.org) established a comprehensive, unique multicentre biobank of human islets and pancreas tissues from organ donors and metabolically phenotyped pancreatectomised patients (PPP). Methods Affymetrix microarrays were used to assess the islet transcriptome of islets isolated either by enzymatic digestion from 103 organ donors (OD), including 84 non-diabetic and 19 type 2 diabetic individuals, or by laser capture microdissection (LCM) from surgical specimens of 103 PPP, including 32 non-diabetic, 36 with type 2 diabetes, 15 with impaired glucose tolerance (IGT) and 20 with recent-onset diabetes (
- Subjects :
- Male
0301 basic medicine
Beta cell
Biobank
Diabetes
Gene expression
Insulin secretion
Islet
Laser capture microdissection
Organ donor
Pancreatectomy
Systems biology
Internal Medicine
Endocrinology, Diabetes and Metabolism
Type 2 diabetes
ddc:616.07
Impaired glucose tolerance
Endocrinology
1114 Paediatrics And Reproductive Medicine
Biological Specimen Banks
Aged, 80 and over
geography.geographical_feature_category
Tissue Donors
3. Good health
HNF1A
Aged
Computational Biology
Diabetes Mellitus, Type 2/metabolism
Female
Humans
Systems Biology/methods
Transcriptome/genetics
Diabetes and Metabolism
medicine.anatomical_structure
1117 Public Health And Health Services
PDX1
Pancreas
endocrine system
medicine.medical_specialty
Biology
Article
Endocrinology & Metabolism
03 medical and health sciences
Internal medicine
Diabetes mellitus
Beta-Zelle, Biobank, Diabetes, Mikrodissektion durch Laser-Capture, Inselchen, Insulinsekretion
medicine
ddc:610
geography
Beta Cell
Gene Expression
Insulin Secretion
Laser Capture Microdissection
Organ Donor
Systems Biology
1103 Clinical Sciences
medicine.disease
beta cell, biobank, diabetes, laser capture microdissection, islet, insulin secretion
030104 developmental biology
Diabetes Mellitus, Type 2
Immunology
Transcriptome
Subjects
Details
- Language :
- English
- ISSN :
- 0012186X
- Database :
- OpenAIRE
- Journal :
- Diabetologia 61, 641–657 (2018), Diabetologia, Diabetologia, vol. 61, no. 3, pp. 641-657, Diabetologia, Vol. 61, No 3 (2018) pp. 641-657
- Accession number :
- edsair.doi.dedup.....bed68b9192f383629adabf1feea42352