Back to Search
Start Over
Increased IVF pregnancy rates after microarray preimplantation genetic diagnosis due to parental translocations
- Source :
- Systems Biology in Reproductive Medicine. 60:119-124
- Publication Year :
- 2013
- Publisher :
- Informa UK Limited, 2013.
-
Abstract
- We successfully performed preimplantation genetic diagnosis (PGD) and simultaneous preimplantation genetic screening (PGS) using single nucleotide polymorphism (SNP) microarrays for couples with balanced chromosome rearrangements in China. A total of 428 molecular karyotypes were diagnosed from 62 couples undergoing 68 in vitro fertilization (IVF) cycles. Of these, 48.1% of the embryos were chromosomally normal without translocation errors or aneuploidy. Of the 428 total embryos, 18.0% embryos were euploid, but were imbalanced due to the transmission of single translocation chromosome derivatives. A total of 6.5% of the embryos had chromosome abnormalities involving the parental chromosome aberration and other chromosomes aneuploidies. Significantly, 27.4% of the embryos were normal/balanced for the rearranged chromosomes, but were abnormal due to aneuploidy affecting other chromosomes. When evaluated on a per IVF cycle basis, 84% of the cycles had at least one chromosomally normal embryo available for uterine transfer. The clinical pregnancy rate per IVF cycle was 54%. Diagnosing genomically balanced embryos through 24 chromosome SNP microarray PGD/PGS, rather than minimally targeted fluorescence in situ hybridization (FISH), is a promising strategy to maximize the pregnancy potential of patients with known parental chromosomal translocations. Moreover, this is the first study to report the clinical application of SNP arrays to screen all 24 chromosome pairs of blastomeres and trophectoderm cells from patients carrying reciprocal translocations in China.
- Subjects :
- Male
Genetics
Preimplantation genetic haplotyping
animal structures
Pregnancy Rate
Urology
Aneuploidy
Chromosome
Chromosomal translocation
Karyotype
Fertilization in Vitro
Biology
Preimplantation genetic diagnosis
medicine.disease
Translocation, Genetic
Reproductive Medicine
Pregnancy
embryonic structures
medicine
Humans
Female
Ploidy
Preimplantation Diagnosis
SNP array
Subjects
Details
- ISSN :
- 19396376 and 19396368
- Volume :
- 60
- Database :
- OpenAIRE
- Journal :
- Systems Biology in Reproductive Medicine
- Accession number :
- edsair.doi.dedup.....beeaa68ed81e8503d7bb07762a03ff06