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T cell receptor β‐chain repertoire analysis of tumor‐infiltrating lymphocytes in pancreatic cancer
- Source :
- Cancer Science
- Publication Year :
- 2018
- Publisher :
- John Wiley and Sons Inc., 2018.
-
Abstract
- Pancreatic cancer is lethal due to lack of perceptible symptoms and effective treatment methods. Immunotherapy may provide promising therapeutic choices for malignant tumors like pancreatic cancer. Tumor-infiltrating lymphocytes (TIL) in tumor mesenchyme could recognize peptide antigens presented on the surface of tumor cells. The present study aimed to test the relationship between the T cell receptor (TCR) β repertoire of the tumor and peripheral blood, and also to investigate the intra-tumor spatial heterogeneity of the TCR β repertoire in pancreatic cancer. To the best of our knowledge, this is the first study to evaluate the clonal composition of TCR β repertoire in TIL across the spatial extent of pancreatic cancer. In this study, we studied 5 patients who were diagnosed with primary pancreatic cancer. Ultra-deep sequencing was used to assess the rearrangement of the TCR β-chain (TCR β) gene. HE staining and immunohistochemistry of CD3, CD4, CD8 and HLA class I were used to show histopathology and immune conditions macroscopically. TIL repertoire showed that different regions of the same tumor showed a greater number of repertoire overlaps between each other than between peripheral blood, which suggested that T cell clones in pancreatic cancer might be quite different from those in peripheral blood. In contrast, intra-tumoral TCR β repertoires were spatially homogeneous between different regions of a single tumor tissue. Based on these results, we speculated that the cellular adaptive immune response in pancreatic cancer was spatially homogeneous; this may pave the way for immunotherapy for the treatment of pancreatic cancer patients.
- Subjects :
- 0301 basic medicine
Male
Cancer Research
intra‐tumor spatial heterogeneity
medicine.medical_treatment
CD3
T cell
Receptors, Antigen, T-Cell, alpha-beta
T-Lymphocytes
pancreatic cancer
chemical and pharmacologic phenomena
Biology
03 medical and health sciences
0302 clinical medicine
Immune system
Basic and Clinical Immunology
Lymphocytes, Tumor-Infiltrating
Pancreatic cancer
medicine
Humans
Aged
Tumor-infiltrating lymphocytes
T-cell receptor
High-Throughput Nucleotide Sequencing
General Medicine
Immunotherapy
Original Articles
Middle Aged
medicine.disease
Complementarity Determining Regions
Clone Cells
Pancreatic Neoplasms
030104 developmental biology
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
immunohistochemistry
biology.protein
Cancer research
Original Article
Female
T cell receptor
ultra‐deep sequencing
CD8
Subjects
Details
- Language :
- English
- ISSN :
- 13497006 and 13479032
- Volume :
- 110
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cancer Science
- Accession number :
- edsair.doi.dedup.....beee7c4ae44a426a9f47d1153d67ec0e