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Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck
- Source :
- Nanomaterials, Volume 9, Issue 10, Nanomaterials, Vol 9, Iss 10, p 1378 (2019)
- Publication Year :
- 2019
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2019.
-
Abstract
- Intraoperative definition of tumor free resection margins in head and neck cancer is challenging. In the current proof-of-principle study we evaluated a novel silica nanoparticle-based agent for its potential use as contrast enhancer. We synthesized silica nanoparticles with an average size of 45 nm and modified these particles with the fluorescence stain fluorescein isocyanate (FITC) for particle detection and with epidermal growth factor receptor (EGFR)-targeting antibodies for enhanced tumor specificity. The nanoparticles exhibited good biocompatibility and could be detected in vitro and in vivo by confocal laser scanning microscopy. Additionally, we show in an ex vivo setting that these modified nanoparticles specifically bind to tumor samples and could be detected using a handheld confocal fluorescence endomicroscope. From a clinical point of view, we believe that this method could be used for tumor border contrast enhancement and for better intraoperative definition of R-0 tumor resection.
- Subjects :
- biology
Biocompatibility
General Chemical Engineering
Confocal
EGFR
contrast agent
silica nanoparticles
Stain
EGFR Antibody
Article
lcsh:Chemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
chemistry
lcsh:QD1-999
In vivo
030220 oncology & carcinogenesis
biology.protein
General Materials Science
Epidermal growth factor receptor
Fluorescein
030223 otorhinolaryngology
Ex vivo
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 20794991
- Database :
- OpenAIRE
- Journal :
- Nanomaterials
- Accession number :
- edsair.doi.dedup.....befb813d5d4487164b0c44642f075a6f
- Full Text :
- https://doi.org/10.3390/nano9101378