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Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck

Authors :
Jan Gosepath
Ralf Kiesslich
Wolfgang Tremel
Anna-Maria Bauer
Juergen Brieger
Rita Gieringer
Anna Watermann
Sven Kurch
Source :
Nanomaterials, Volume 9, Issue 10, Nanomaterials, Vol 9, Iss 10, p 1378 (2019)
Publication Year :
2019
Publisher :
Multidisciplinary Digital Publishing Institute, 2019.

Abstract

Intraoperative definition of tumor free resection margins in head and neck cancer is challenging. In the current proof-of-principle study we evaluated a novel silica nanoparticle-based agent for its potential use as contrast enhancer. We synthesized silica nanoparticles with an average size of 45 nm and modified these particles with the fluorescence stain fluorescein isocyanate (FITC) for particle detection and with epidermal growth factor receptor (EGFR)-targeting antibodies for enhanced tumor specificity. The nanoparticles exhibited good biocompatibility and could be detected in vitro and in vivo by confocal laser scanning microscopy. Additionally, we show in an ex vivo setting that these modified nanoparticles specifically bind to tumor samples and could be detected using a handheld confocal fluorescence endomicroscope. From a clinical point of view, we believe that this method could be used for tumor border contrast enhancement and for better intraoperative definition of R-0 tumor resection.

Details

Language :
English
ISSN :
20794991
Database :
OpenAIRE
Journal :
Nanomaterials
Accession number :
edsair.doi.dedup.....befb813d5d4487164b0c44642f075a6f
Full Text :
https://doi.org/10.3390/nano9101378